2q9e

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{{STRUCTURE_2q9e| PDB=2q9e | SCENE= }}
{{STRUCTURE_2q9e| PDB=2q9e | SCENE= }}
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'''Structure of spin-labeled T4 lysozyme mutant S44R1'''
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===Structure of spin-labeled T4 lysozyme mutant S44R1===
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==Overview==
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Site-directed spin labeling provides a means for exploring structure and dynamics in proteins. To interpret the complex EPR spectra that often arise, it is necessary to characterize the rotamers of the spin-labeled side chain and the interactions they make with the local environment in proteins of known structure. For this purpose, crystal structures have been determined for T4 lysozyme bearing a nitroxide side chain (R1) at the solvent-exposed helical sites 41 and 44 in the B helix. These sites are of particular interest in that the corresponding EPR spectra reveal two dynamic states of R1, one of which is relatively immobilized suggesting interactions of the nitroxide with the environment. The crystal structures together with the effect of mutagenesis of nearest neighbors on the motion of R1 suggest intrahelical interactions of 41R1 with the i + 4 residue and of 44R1 with the i + 1 residue. Such interactions appear to be specific to particular rotamers of the R1 side chain.
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(as it appears on PubMed at http://www.pubmed.gov), where 18096642 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18096642}}
==About this Structure==
==About this Structure==
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[[Category: Spin label]]
[[Category: Spin label]]
[[Category: T4 lysozyme]]
[[Category: T4 lysozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:34:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:12:36 2008''

Revision as of 09:12, 28 July 2008

Image:2q9e.png

Template:STRUCTURE 2q9e

Structure of spin-labeled T4 lysozyme mutant S44R1

Template:ABSTRACT PUBMED 18096642

About this Structure

2Q9E is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Structural determinants of nitroxide motion in spin-labeled proteins: Solvent-exposed sites in helix B of T4 lysozyme., Guo Z, Cascio D, Hideg K, Hubbell WL, Protein Sci. 2008 Feb;17(2):228-39. Epub 2007 Dec 20. PMID:18096642

Page seeded by OCA on Mon Jul 28 12:12:36 2008

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