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1n8e

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{{STRUCTURE_1n8e| PDB=1n8e | SCENE= }}
{{STRUCTURE_1n8e| PDB=1n8e | SCENE= }}
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'''Fragment Double-D from Human Fibrin'''
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===Fragment Double-D from Human Fibrin===
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==Overview==
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The crystal structure of fragment double-D from factor XIII-cross-linked lamprey fibrin has been determined at 2.9 A resolution. The 180 kDa covalent dimer was cocrystallized with the peptide Gly-His-Arg-Pro-amide, which in many fibrinogens, but not that of lamprey, corresponds to the B-knob exposed by thrombin. The structure was determined by molecular replacement, a recently determined structure of lamprey fragment D being used as a search model. GHRPam was found in both the gamma- and beta-chain holes. Unlike the situation with fragment D, the crystal packing of the cross-linked double-D structure exhibits two different D-D interfaces, each gamma-chain facing gamma-chains on two other molecules. One of these (interface I) involves the asymmetric interface observed in all other D fragments and related structures. The other (interface II) encompasses a completely different set of residues. The two abutments differ in that interface I results in an "in line" arrangement of abutting molecules and the interface II in a "zigzag" arrangement. So far as can be determined (the electron density could only be traced on one side of the cross-links), it is the gamma-chains of the newly observed zigzag units (interface II) that are joined by the reciprocal epsilon-amino-gamma-glutamyl cross-links. Auspiciously, the same novel D-D interface was observed in two lower-resolution crystal structures of human double-D preparations that had been crystallized under unusual circumstances. These observations show that double-D structures are linked in a way that is sufficiently flexible to accommodate different D-D interfaces under different circumstances.
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==About this Structure==
==About this Structure==
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[[Category: Crystal packing]]
[[Category: Crystal packing]]
[[Category: D:d interface]]
[[Category: D:d interface]]
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Revision as of 09:13, 28 July 2008

Image:1n8e.png

Template:STRUCTURE 1n8e

Fragment Double-D from Human Fibrin

Template:ABSTRACT PUBMED 12501189

About this Structure

1N8E is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The crystal structure of fragment double-D from cross-linked lamprey fibrin reveals isopeptide linkages across an unexpected D-D interface., Yang Z, Pandi L, Doolittle RF, Biochemistry. 2002 Dec 31;41(52):15610-7. PMID:12501189

Page seeded by OCA on Mon Jul 28 12:13:12 2008

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