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1tbk

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[[Image:1tbk.gif|left|200px]]
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{{STRUCTURE_1tbk| PDB=1tbk | SCENE= }}
{{STRUCTURE_1tbk| PDB=1tbk | SCENE= }}
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'''NMR structure of the VS ribozyme stem-loop V RNA in the absence of multivalent ions.'''
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===NMR structure of the VS ribozyme stem-loop V RNA in the absence of multivalent ions.===
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==Overview==
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An important step in the substrate recognition of the Neurospora Varkud Satellite (VS) ribozyme is the formation of a magnesium-dependent loop/loop interaction between the terminal loops of stem-loops I and V. We have studied the structure of stem-loop V by nuclear magnetic resonance spectroscopy and shown that it adopts a U-turn conformation, a common motif found in RNA. Structural comparisons indicate that the U-turn of stem-loop V fulfills some but not all of the structural characteristics found in canonical U-turn structures. This U-turn conformation exposes the Watson-Crick faces of the bases within stem-loop V (G697, A698, and C699) and makes them accessible for interaction with stem-loop I. Using chemical-shift mapping, we show that magnesium ions interact with the loop of the isolated stem-loop V and induce a conformational change that may be important for interaction with stem-loop I. This study expands our understanding of the role of U-turn motifs in RNA structure and function and provides insights into the mechanism of substrate recognition in the VS ribozyme.
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The line below this paragraph, {{ABSTRACT_PUBMED_15766243}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15766243 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15766243}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TBK OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TBK OCA].
==Reference==
==Reference==
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[[Category: Hairpin]]
[[Category: Hairpin]]
[[Category: U-turn]]
[[Category: U-turn]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:46:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:44:43 2008''

Revision as of 09:44, 28 July 2008

Image:1tbk.png

Template:STRUCTURE 1tbk

NMR structure of the VS ribozyme stem-loop V RNA in the absence of multivalent ions.

Template:ABSTRACT PUBMED 15766243

About this Structure

Full experimental information is available from OCA.

Reference

Nuclear magnetic resonance structure of the Varkud satellite ribozyme stem-loop V RNA and magnesium-ion binding from chemical-shift mapping., Campbell DO, Legault P, Biochemistry. 2005 Mar 22;44(11):4157-70. PMID:15766243

Page seeded by OCA on Mon Jul 28 12:44:43 2008

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