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2jxm

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{{STRUCTURE_2jxm| PDB=2jxm | SCENE= }}
{{STRUCTURE_2jxm| PDB=2jxm | SCENE= }}
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'''Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex'''
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===Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex===
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==Overview==
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The nature of transient protein complexes can range from a highly dynamic ensemble of orientations to a single well-defined state. This represents variation in the equilibrium between the encounter and final, functional state. The transient complex between plastocyanin (Pc) and cytochrome f (cytf) of the cyanobacterium Prochlorothrix hollandica was characterized by NMR spectroscopy. Intermolecular pseudocontact shifts and chemical shift perturbations were used as restraints in docking calculations to determine the structure of the wild-type Pc-cytf complex. The orientation of Pc is similar to orientations found in Pc-cytf complexes from other sources. Electrostatics seems to play a modest role in complex formation. A large variability in the ensemble of lowest energy structures indicates a dynamic nature of the complex. Two unusual hydrophobic patch residues in Pc have been mutated to the residues found in other plastocyanins (Y12G/P14L). The binding constants are similar for the complexes of cytf with wild-type Pc and mutant Pc, but the chemical shift perturbations are smaller for the complex with mutant Pc. Docking calculations for the Y12G/P14L Pc-cytf complex did not produce a converged ensemble of structures. Simulations of the dynamics were performed using the observed averaged NMR parameters as input. The results indicate a surprisingly large amplitude of mobility of Y12G/P14L Pc within the complex. It is concluded that the double mutation shifts the complex further from the well-defined toward the encounter state.
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{{ABSTRACT_PUBMED_18201089}}
==About this Structure==
==About this Structure==
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2JXM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Prochlorothrix_hollandica Prochlorothrix hollandica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXM OCA].
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2JXM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Prochlorothrix_hollandica Prochlorothrix hollandica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXM OCA].
==Reference==
==Reference==
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[[Category: Metal-binding]]
[[Category: Metal-binding]]
[[Category: Transport]]
[[Category: Transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:22:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:56:34 2008''

Revision as of 09:56, 28 July 2008

Image:2jxm.png

Template:STRUCTURE 2jxm

Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex

Template:ABSTRACT PUBMED 18201089

About this Structure

2JXM is a Protein complex structure of sequences from Prochlorothrix hollandica. Full experimental information is available from OCA.

Reference

Dynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica., Hulsker R, Baranova MV, Bullerjahn GS, Ubbink M, J Am Chem Soc. 2008 Feb 13;130(6):1985-91. Epub 2008 Jan 18. PMID:18201089

Page seeded by OCA on Mon Jul 28 12:56:34 2008

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