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1p4x

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{{STRUCTURE_1p4x| PDB=1p4x | SCENE= }}
{{STRUCTURE_1p4x| PDB=1p4x | SCENE= }}
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'''Crystal structure of SarS protein from Staphylococcus Aureus'''
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===Crystal structure of SarS protein from Staphylococcus Aureus===
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==Overview==
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The expression of virulence determinants in Staphylococcus aureus is controlled by global regulatory loci (e.g., sarA and agr). One of these determinants, protein A (spa), is activated by sarS, which encodes a 250-residue DNA-binding protein. Genetic analysis indicated that the agr locus likely mediates spa repression by suppressing the transcription of sarS. Contrary to SarA and SarR, which require homodimer formation for proper function, SarS is unusual within the SarA protein family in that it contains two homologous halves, with each half sharing sequence similarity to SarA and SarR. Here we report the 2.2 A resolution X-ray crystal structure of the SarS protein. SarS has folds similar to those of SarR and, quite plausibly, the native SarA structure. Two typical winged-helix DNA-binding domains are connected by a well-ordered loop. The interactions between the two domains are extensive and conserved. The putative DNA-binding surface is highly positively charged. In contrast, negatively charged patches are located opposite to the DNA-binding surface. Furthermore, sequence alignment and structural comparison revealed that MarR has folds similar to those of SarR and SarS. Members of the MarR protein family have previously been implicated in the negative regulation of an efflux pump involved in multiple antibiotic resistance in many gram-negative species. We propose that MarR also belongs to the winged-helix protein family and has a similar mode of DNA binding as SarR and SarS and possibly the entire SarA protein family member. Based on the structural differences of SarR, SarS, and MarR, we further classified these winged-helix proteins to three subfamilies, SarA, SarS, and MarR. Finally, a possible transcription regulation mechanism is proposed.
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{{ABSTRACT_PUBMED_12837797}}
==About this Structure==
==About this Structure==
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[[Category: Zhang, G.]]
[[Category: Zhang, G.]]
[[Category: Winged-helix protein]]
[[Category: Winged-helix protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:33:38 2008''

Revision as of 10:33, 28 July 2008

Image:1p4x.png

Template:STRUCTURE 1p4x

Crystal structure of SarS protein from Staphylococcus Aureus

Template:ABSTRACT PUBMED 12837797

About this Structure

1P4X is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the SarS protein from Staphylococcus aureus., Li R, Manna AC, Dai S, Cheung AL, Zhang G, J Bacteriol. 2003 Jul;185(14):4219-25. PMID:12837797

Page seeded by OCA on Mon Jul 28 13:33:38 2008

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