This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3bj9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:3bj9.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:3bj9.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_3bj9| PDB=3bj9 | SCENE= }}
{{STRUCTURE_3bj9| PDB=3bj9 | SCENE= }}
-
'''Crystal structure of the Surrogate Light Chain Variable Domain VpreBJ'''
+
===Crystal structure of the Surrogate Light Chain Variable Domain VpreBJ===
-
==Overview==
+
<!--
-
The surrogate light chain (SLC) is a key regulator of B cell development in the bone marrow, resulting in mature B cells that produce antibodies that are capable of interacting with antigens. The SLC comprises two noncovalently interacting proteins: VpreB and 14.1. We engineered a construct to represent the complete immunoglobulin-like domain of the SLC variable domain in a single protein chain that could be bacterially expressed. In this construct, the incomplete immunoglobulin domain of VpreB (residues 1-102) was linked to the J-segment of 14.1 (residues 40-53), which provided one beta-strand to complete the V-like domain (VpreBJ). Because VpreBJ has the interface to VH chains, but lacks the unique region of 14.1, which is important for SLC signaling, we predict that a properly folded VpreBJ would have the potential to act as a dominant negative mutant of the surrogate light chain. X-ray crystallography of VpreBJ at 2.0 A resolution showed that the engineering was successful. With its two beta-pleated sheets, packed face-to-face, the single chain VpreBJ resembles a mature light chain immunoglobulin V-domain (VL). The surface that would normally interact with the VH chain interacts with a crystallographically related VpreBJ molecule. The presence of dimeric species in solution was verified by analytical ultracentrifugation. VpreBJ is easily overexpressed in bacteria, while retaining the native conformation of an immunoglobulin domain, and thus may serve as an important reagent for future studies in B-cell development.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18287279}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18287279 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18287279}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Immunoglobulin domain]]
[[Category: Immunoglobulin domain]]
[[Category: Polymorphism]]
[[Category: Polymorphism]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:50:25 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:38:51 2008''

Revision as of 10:38, 28 July 2008

Image:3bj9.png

Template:STRUCTURE 3bj9

Crystal structure of the Surrogate Light Chain Variable Domain VpreBJ

Template:ABSTRACT PUBMED 18287279

About this Structure

3BJ9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Engineering and characterization of a single chain surrogate light chain variable domain., Morstadt L, Bohm A, Yuksel D, Kumar K, Stollar BD, Baleja JD, Protein Sci. 2008 Mar;17(3):458-65. PMID:18287279

Page seeded by OCA on Mon Jul 28 13:38:51 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bj9"
Personal tools