1vtk

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{{STRUCTURE_1vtk| PDB=1vtk | SCENE= }}
{{STRUCTURE_1vtk| PDB=1vtk | SCENE= }}
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'''THYMIDINE KINASE FROM HERPES SIMPLEX VIRUS TYPE 1 IN COMPLEX WITH ADP AND DEOXYTHYMIDINE-MONOPHOSPHATE'''
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===THYMIDINE KINASE FROM HERPES SIMPLEX VIRUS TYPE 1 IN COMPLEX WITH ADP AND DEOXYTHYMIDINE-MONOPHOSPHATE===
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==Overview==
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Thymidine kinase from Herpes simplex virus type 1 (TK) was crystallized in an N-terminally truncated but fully active form. The structures of TK complexed with ADP at the ATP-site and deoxythymidine-5'-monophosphate (dTMP), deoxythymidine (dT), or idoxuridine-5'-phosphate (5-iodo-dUMP) at the substrate-site were refined to 2.75 A, 2.8 A, and 3.0 A resolution, respectively. TK catalyzes the phosphorylation of dT resulting in an ester, and the phosphorylation of dTMP giving rise to an anhydride. The presented TK structures indicate that there are only small differences between these two modes of action. Glu83 serves as a general base in the ester reaction. Arg163 parks at an internal aspartate during ester formation and binds the alpha-phosphate of dTMP during anhydride formation. The bound deoxythymidine leaves a 35 A3 cavity at position 5 of the base and two sequestered water molecules at position 2. Cavity and water molecules reduce the substrate specificity to such an extent that TK can phosphorylate various substrate analogues useful in pharmaceutical applications. TK is structurally homologous to the well-known nucleoside monophosphate kinases but contains large additional peptide segments.
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(as it appears on PubMed at http://www.pubmed.gov), where 9336833 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9336833}}
==About this Structure==
==About this Structure==
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[[Category: Target for anti-herpes viral drug]]
[[Category: Target for anti-herpes viral drug]]
[[Category: Transferase]]
[[Category: Transferase]]
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Revision as of 10:45, 28 July 2008

Image:1vtk.png

Template:STRUCTURE 1vtk

THYMIDINE KINASE FROM HERPES SIMPLEX VIRUS TYPE 1 IN COMPLEX WITH ADP AND DEOXYTHYMIDINE-MONOPHOSPHATE

Template:ABSTRACT PUBMED 9336833

About this Structure

1VTK is a Single protein structure of sequence from Human herpesvirus 1. Full crystallographic information is available from OCA.

Reference

The structures of thymidine kinase from herpes simplex virus type 1 in complex with substrates and a substrate analogue., Wild K, Bohner T, Folkers G, Schulz GE, Protein Sci. 1997 Oct;6(10):2097-106. PMID:9336833

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