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2ewe

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{{STRUCTURE_2ewe| PDB=2ewe | SCENE= }}
{{STRUCTURE_2ewe| PDB=2ewe | SCENE= }}
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'''Crystal structure of Pectate Lyase C R218K mutant in complex with pentagalacturonic acid'''
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===Crystal structure of Pectate Lyase C R218K mutant in complex with pentagalacturonic acid===
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==Overview==
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The three-dimensional structure of a complex between the pectate lyase C (PelC) R218K mutant and a plant cell wall fragment has been determined by x-ray diffraction techniques to a resolution of 2.2 A and refined to a crystallographic R factor of 18.6%. The oligosaccharide substrate, alpha-D-GalpA-([1--&gt;4]-alpha-D-GalpA)3-(1--&gt;4)-D-GalpA , is composed of five galacturonopyranose units (D-GalpA) linked by alpha-(1--&gt;4) glycosidic bonds. PelC is secreted by the plant pathogen Erwinia chrysanthemi and degrades the pectate component of plant cell walls in soft rot diseases. The substrate has been trapped in crystals by using the inactive R218K mutant. Four of the five saccharide units of the substrate are well ordered and represent an atomic view of the pectate component in plant cell walls. The conformation of the pectate fragment is a mix of 21 and 31 right-handed helices. The substrate binds in a cleft, interacting primarily with positively charged groups: either lysine or arginine amino acids on PelC or the four Ca2+ ions found in the complex. The observed protein-oligosaccharide interactions provide a functional explanation for many of the invariant and conserved amino acids in the pectate lyase family of proteins. Because the R218K PelC-galacturonopentaose complex represents an intermediate in the reaction pathway, the structure also reveals important details regarding the enzymatic mechanism. Notably, the results suggest that an arginine, which is invariant in the pectate lyase superfamily, is the amino acid that initiates proton abstraction during the beta elimination cleavage of polygalacturonic acid.
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==About this Structure==
==About this Structure==
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[[Category: Parallel beta helix]]
[[Category: Parallel beta helix]]
[[Category: Protein-oligosaccharide interaction]]
[[Category: Protein-oligosaccharide interaction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:11:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:56:17 2008''

Revision as of 10:56, 28 July 2008

Image:2ewe.png

Template:STRUCTURE 2ewe

Crystal structure of Pectate Lyase C R218K mutant in complex with pentagalacturonic acid

Template:ABSTRACT PUBMED 10368179

About this Structure

2EWE is a Single protein structure of sequence from Erwinia chrysanthemi. Full crystallographic information is available from OCA.

Reference

Structure of a plant cell wall fragment complexed to pectate lyase C., Scavetta RD, Herron SR, Hotchkiss AT, Kita N, Keen NT, Benen JA, Kester HC, Visser J, Jurnak F, Plant Cell. 1999 Jun;11(6):1081-92. PMID:10368179

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