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1x27

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{{STRUCTURE_1x27| PDB=1x27 | SCENE= }}
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'''Crystal Structure of Lck SH2-SH3 with SH2 binding site of p130Cas'''
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===Crystal Structure of Lck SH2-SH3 with SH2 binding site of p130Cas===
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==Overview==
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The docking protein p130Cas (Cas) becomes tyrosine-phosphorylated in its central substrate domain in response to extracellular stimuli such as integrin-mediated cell adhesion, and transmits signals through interactions with various intracellular signaling molecules such as the adaptor protein Crk. Src-family kinases (SFKs) bind a specific site in the carboxyl-terminal region of Cas and subsequently SFKs phosphorylate progressively the substrate domain in Cas. In this study crystallography, mutagenesis and binding assays were used to understand the molecular basis for Cas interactions with SFKs. Tyrosine phosphorylation regulates binding of Cas to SFKs, and the primary site for this phosphorylation, Y762, has been proposed. A phosphorylated peptide corresponding to Cas residues 759MEDpYDYVHL767 containing the key phosphotyrosine was crystallized in complex with the SH3-SH2 domain of the SFK Lck. The results provide the first structural data for this protein-protein interaction. The motif in Cas 762pYDYV binds to the SH2 domain in a mode that mimics high-affinity ligands, involving dual contacts of Y762 and V765 with conserved residues in SFK SH2 domains. In addition, Y764 is in position to make an electrostatic contact after phosphorylation with a conserved SFK arginine that mediates interactions with other high-affinity SH2 binders. These new molecular data suggest that Cas may regulate activity of Src as a competing ligand to displace intramolecular interactions that occur in SFKs (between the C-terminal tail and the SH2 domain) and restrain and down-regulate the kinase in an inactive form.
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(as it appears on PubMed at http://www.pubmed.gov), where 16245368 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16245368}}
==Disease==
==Disease==
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[[Category: Lck phospho-peptide complex]]
[[Category: Lck phospho-peptide complex]]
[[Category: Lck-cas complex]]
[[Category: Lck-cas complex]]
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Revision as of 11:09, 28 July 2008

Image:1x27.png

Template:STRUCTURE 1x27

Contents

Crystal Structure of Lck SH2-SH3 with SH2 binding site of p130Cas

Template:ABSTRACT PUBMED 16245368

Disease

Known disease associated with this structure: SCID due to LCK deficiency OMIM:[153390]

About this Structure

1X27 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Molecular basis for regulation of Src by the docking protein p130Cas., Nasertorabi F, Tars K, Becherer K, Kodandapani R, Liljas L, Vuori K, Ely KR, J Mol Recognit. 2006 Jan-Feb;19(1):30-8. PMID:16245368

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