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1x9h

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{{STRUCTURE_1x9h| PDB=1x9h | SCENE= }}
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'''Crystal structure of phosphoglucose/phosphomannose isomerase from Pyrobaculum aerophilum in complex with fructose 6-phosphate'''
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===Crystal structure of phosphoglucose/phosphomannose isomerase from Pyrobaculum aerophilum in complex with fructose 6-phosphate===
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==Overview==
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The crystal structure of a dual-specificity phosphoglucose/phosphomannose isomerase from the crenarchaeon Pyrobaculum aerophilum (PaPGI/PMI) has been determined in complex with glucose 6-phosphate at 1.16 A resolution and with fructose 6-phosphate at 1.5 A resolution. Subsequent modeling of mannose 6-phosphate (M6P) into the active site of the enzyme shows that the PMI activity of this enzyme may be due to the additional space imparted by a threonine. In PGIs from bacterial and eukaryotic sources, which cannot use M6P as a substrate, the equivalent residue is a glutamine. The increased space may permit rotation of the C2-C3 bond in M6P to facilitate abstraction of a proton from C2 by Glu203 and, after a further C2-C3 rotation of the resulting cis-enediolate, re-donation of a proton to C1 by the same residue. A proline residue (in place of a glycine in PGI) may also promote PMI activity by positioning the C1-O1 region of M6P. Thus, the PMI reaction in PaPGI/PMI probably uses a cis-enediol mechanism of catalysis, and this activity appears to arise from a subtle difference in the architecture of the enzyme, compared to bacterial and eukaryotic PGIs.
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(as it appears on PubMed at http://www.pubmed.gov), where 15518558 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15518558}}
==About this Structure==
==About this Structure==
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[[Category: Hyperthermophile]]
[[Category: Hyperthermophile]]
[[Category: Pgi superfamily]]
[[Category: Pgi superfamily]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:44:43 2008''
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Revision as of 12:02, 28 July 2008

Image:1x9h.png

Template:STRUCTURE 1x9h

Crystal structure of phosphoglucose/phosphomannose isomerase from Pyrobaculum aerophilum in complex with fructose 6-phosphate

Template:ABSTRACT PUBMED 15518558

About this Structure

1X9H is a Single protein structure of sequence from Pyrobaculum aerophilum. Full crystallographic information is available from OCA.

Reference

Structural basis for phosphomannose isomerase activity in phosphoglucose isomerase from Pyrobaculum aerophilum: a subtle difference between distantly related enzymes., Swan MK, Hansen T, Schonheit P, Davies C, Biochemistry. 2004 Nov 9;43(44):14088-95. PMID:15518558

Page seeded by OCA on Mon Jul 28 15:02:40 2008

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