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1w4o

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[[Image:1w4o.gif|left|200px]]
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{{Seed}}
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[[Image:1w4o.png|left|200px]]
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{{STRUCTURE_1w4o| PDB=1w4o | SCENE= }}
{{STRUCTURE_1w4o| PDB=1w4o | SCENE= }}
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'''BINDING OF NONNATURAL 3'-NUCLEOTIDES TO RIBONUCLEASE A'''
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===BINDING OF NONNATURAL 3'-NUCLEOTIDES TO RIBONUCLEASE A===
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==Overview==
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2'-Fluoro-2'-deoxyuridine 3'-phosphate (dU(F)MP) and arabinouridine 3'-phosphate (araUMP) have non-natural furanose rings. dU(F)MP and araUMP were prepared by chemical synthesis and found to have three- to sevenfold higher affinity than uridine 3'-phosphate (3'-UMP) or 2'-deoxyuridine 3'-phosphate (dUMP) for ribonuclease A (RNase A). These differences probably arise (in part) from the phosphoryl groups of 3'-UMP, dU(F)MP, and araUMP (pK(a) = 5.9) being more anionic than that of dUMP (pK(a) = 6.3). The three-dimensional structures of the crystalline complexes of RNase A with dUMP, dU(F)MP and araUMP were determined at &lt; 1.7 A resolution by X-ray diffraction analysis. In these three structures, the uracil nucleobases and phosphoryl groups bind to the enzyme in a nearly identical position. Unlike 3'-UMP and dU(F)MP, dUMP and araUMP bind with their furanose rings in the preferred pucker. In the RNase A.araUMP complex, the 2'-hydroxyl group is exposed to the solvent. All four 3'-nucleotides bind more tightly to wild-type RNase A than to its T45G variant, which lacks the residue that interacts most closely with the uracil nucleobase. These findings illuminate in atomic detail the interaction of RNase A and 3'-nucleotides, and indicate that non-natural furanose rings can serve as the basis for more potent inhibitors of catalysis by RNase A.
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(as it appears on PubMed at http://www.pubmed.gov), where 15670155 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15670155}}
==About this Structure==
==About this Structure==
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[[Category: Nuclease]]
[[Category: Nuclease]]
[[Category: Ribonuclease some]]
[[Category: Ribonuclease some]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:09:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:18:16 2008''

Revision as of 12:18, 28 July 2008

Image:1w4o.png

Template:STRUCTURE 1w4o

BINDING OF NONNATURAL 3'-NUCLEOTIDES TO RIBONUCLEASE A

Template:ABSTRACT PUBMED 15670155

About this Structure

1W4O is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Binding of non-natural 3'-nucleotides to ribonuclease A., Jenkins CL, Thiyagarajan N, Sweeney RY, Guy MP, Kelemen BR, Acharya KR, Raines RT, FEBS J. 2005 Feb;272(3):744-55. PMID:15670155

Page seeded by OCA on Mon Jul 28 15:18:16 2008

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