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1zsh

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{{STRUCTURE_1zsh| PDB=1zsh | SCENE= }}
{{STRUCTURE_1zsh| PDB=1zsh | SCENE= }}
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'''Crystal structure of bovine arrestin-2 in complex with inositol hexakisphosphate (IP6)'''
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===Crystal structure of bovine arrestin-2 in complex with inositol hexakisphosphate (IP6)===
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==Overview==
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Interactions between arrestins and phosphoinositides have been reported to regulate multiple membrane-associated signaling and trafficking events including clathrin-mediated endocytosis and light adaptation in Drosophila. Arrestins have been proposed to have nuclear and cytosolic functions as well, although the ligand dependence of these functions has not been investigated. Here we characterize the structural, molecular, and cellular interactions between arrestin-2 and inositol hexakisphosphate (inositol 1,2,3,4,5,6-hexakisphosphate (IP(6))). The crystal structure of the arrestin-2.IP(6) complex was solved to 2.9 A with crystal lattice contacts suggesting two sites on a protein monomer mediating IP(6) binding. Mutagenesis coupled to isothermal titration calorimetry and tritiated IP(6) binding assays confirmed two-site binding with a low affinity IP(6)-binding site in the N-domain and a high affinity site in the C-domain. Native gel electrophoresis, gel filtration, and analytical ultracentrifugation demonstrated the ability of IP(6) to promote arrestin-2 oligomerization via the two crystallographically defined ligand-binding locations. In addition, analysis in mammalian cells revealed that arrestin-2 not only undergoes homo-oligomerization, but it can also hetero-oligomerize with arrestin-3 in a manner that depends on IP(6)-binding sites. Mutation of either IP(6)-binding site in arrestin-2 disrupted oligomerization while interactions with known binding partners including clathrin, AP-2, and ERK2 were maintained. Subcellular localization studies showed that arrestin-2 oligomers are primarily cytoplasmic, whereas arrestin-2 monomers displayed increased nuclear localization. Thus, by promoting cytosolic oligomerization, IP(6) binding is proposed to be a negative regulator of interactions of arrestin with plasma membrane and nuclear signaling proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 16439357 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16439357}}
==About this Structure==
==About this Structure==
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[[Category: Nonvisual arrestin]]
[[Category: Nonvisual arrestin]]
[[Category: Signal transduction]]
[[Category: Signal transduction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:01:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:27:27 2008''

Revision as of 12:27, 28 July 2008

Image:1zsh.png

Template:STRUCTURE 1zsh

Crystal structure of bovine arrestin-2 in complex with inositol hexakisphosphate (IP6)

Template:ABSTRACT PUBMED 16439357

About this Structure

1ZSH is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Nonvisual arrestin oligomerization and cellular localization are regulated by inositol hexakisphosphate binding., Milano SK, Kim YM, Stefano FP, Benovic JL, Brenner C, J Biol Chem. 2006 Apr 7;281(14):9812-23. Epub 2006 Jan 26. PMID:16439357

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