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| - | [[Image:1phc.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1phc| PDB=1phc | SCENE= }} | | {{STRUCTURE_1phc| PDB=1phc | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF SUBSTRATE-FREE PSEUDOMONAS PUTIDA CYTOCHROME P450'''
| + | ===CRYSTAL STRUCTURE OF SUBSTRATE-FREE PSEUDOMONAS PUTIDA CYTOCHROME P450=== |
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| - | ==Overview==
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| - | The crystal structure of Pseudomonas putida cytochrome P-450cam in the substrate-free form has been refined at 2.20-A resolution and compared to the substrate-bound form of the enzyme. In the absence of the substrate camphor, the P-450cam heme iron atom is hexacoordinate with the sulfur atom of Cys-357 providing one axial heme ligand and a water molecule or hydroxide ion providing the other axial ligand. A network of hydrogen-bonded solvent molecules occupies the substrate pocket in addition to the iron-linked aqua ligand. When a camphor molecule binds, the active site waters including the aqua ligand are displaced, resulting in a pentacoordinate high-spin heme iron atom. Analysis of the Fno camphor - F camphor difference Fourier and a quantitative comparison of the two refined structures reveal that no detectable conformational change results from camphor binding other than a small repositioning of a phenylalanine side chain that contacts the camphor molecule. However, large decreases in the mean temperature factors of three separate segments of the protein centered on Tyr-96, Thr-185, and Asp-251 result from camphor binding. This indicates that camphor binding decreases the flexibility in these three regions of the P-450cam molecule without altering the mean position of the atoms involved. | + | The line below this paragraph, {{ABSTRACT_PUBMED_3768350}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 3768350 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_3768350}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Single protein]] | | [[Category: Single protein]] |
| | [[Category: Poulos, T L.]] | | [[Category: Poulos, T L.]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:05:23 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:35:51 2008'' |
Revision as of 12:35, 28 July 2008
Template:STRUCTURE 1phc
CRYSTAL STRUCTURE OF SUBSTRATE-FREE PSEUDOMONAS PUTIDA CYTOCHROME P450
Template:ABSTRACT PUBMED 3768350
About this Structure
1PHC is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.
Reference
Crystal structure of substrate-free Pseudomonas putida cytochrome P-450., Poulos TL, Finzel BC, Howard AJ, Biochemistry. 1986 Sep 9;25(18):5314-22. PMID:3768350
Page seeded by OCA on Mon Jul 28 15:35:51 2008
