1xg0

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{{STRUCTURE_1xg0| PDB=1xg0 | SCENE= }}
{{STRUCTURE_1xg0| PDB=1xg0 | SCENE= }}
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'''High resolution crystal structure of phycoerythrin 545 from the marine cryptophyte rhodomonas CS24'''
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===High resolution crystal structure of phycoerythrin 545 from the marine cryptophyte rhodomonas CS24===
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==Overview==
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Cryptophyte algae differ from cyanobacteria and red algae in the architecture of their photosynthetic light harvesting systems, even though all three are evolutionarily related. Central to cryptophyte light harvesting is the soluble antenna protein phycoerythrin 545 (PE545). The ultrahigh resolution crystal structure of PE545, isolated from a unicellular cryptophyte Rhodomonas CS24, is reported at both 1.1A and 0.97A resolution, revealing details of the conformation and environments of the chromophores. Absorption, emission and polarized steady state spectroscopy (298K, 77K), as well as ultrafast (20fs time resolution) measurements of population dynamics are reported. Coupled with complementary quantum chemical calculations of electronic transitions of the bilins, these enable assignment of spectral absorption characteristics to each chromophore in the structure. Spectral differences between the tetrapyrrole pigments due to chemical differences between bilins, as well as their binding and interaction with the local protein environment are described. Based on these assignments, and considering customized optical properties such as strong coupling, a model for light harvesting by PE545 is developed which explains the fast, directional harvesting of excitation energy. The excitation energy is funnelled from four peripheral pigments (beta158,beta82) into a central chromophore dimer (beta50/beta61) in approximately 1ps. Those chromophores, in turn, transfer the excitation energy to the red absorbing molecules located at the periphery of the complex in approximately 4ps. A final resonance energy transfer step sensitizes just one of the alpha19 bilins on a time scale of 22ps. Furthermore, it is concluded that binding of PE545 to the thylakoid membrane is not essential for efficient energy transfer to the integral membrane chlorophyll a-containing complexes associated with PS-II.
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{{ABSTRACT_PUBMED_15504407}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Developing a structure-function model for the cryptophyte phycoerythrin 545 using ultrahigh resolution crystallography and ultrafast laser spectroscopy., Doust AB, Marai CN, Harrop SJ, Wilk KE, Curmi PM, Scholes GD, J Mol Biol. 2004 Nov 12;344(1):135-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15504407 15504407]
Developing a structure-function model for the cryptophyte phycoerythrin 545 using ultrahigh resolution crystallography and ultrafast laser spectroscopy., Doust AB, Marai CN, Harrop SJ, Wilk KE, Curmi PM, Scholes GD, J Mol Biol. 2004 Nov 12;344(1):135-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15504407 15504407]
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Evolution of a light-harvesting protein by addition of new subunits and rearrangement of conserved elements: crystal structure of a cryptophyte phycoerythrin at 1.63-A resolution., Wilk KE, Harrop SJ, Jankova L, Edler D, Keenan G, Sharples F, Hiller RG, Curmi PM, Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8901-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10430868 10430868]
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Phycobilins of cryptophycean algae. Occurrence of dihydrobiliverdin and mesobiliverdin in cryptomonad biliproteins., Wedemayer GJ, Kidd DG, Wemmer DE, Glazer AN, J Biol Chem. 1992 Apr 15;267(11):7315-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1559975 1559975]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Rhodomonas sp.]]
[[Category: Rhodomonas sp.]]
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[[Category: Light-harvesting protein]]
[[Category: Light-harvesting protein]]
[[Category: Phycoerythrin]]
[[Category: Phycoerythrin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:05:49 2008''

Revision as of 13:05, 28 July 2008

Image:1xg0.png

Template:STRUCTURE 1xg0

High resolution crystal structure of phycoerythrin 545 from the marine cryptophyte rhodomonas CS24

Template:ABSTRACT PUBMED 15504407

About this Structure

1XG0 is a Protein complex structure of sequences from Rhodomonas sp.. Full crystallographic information is available from OCA.

Reference

Developing a structure-function model for the cryptophyte phycoerythrin 545 using ultrahigh resolution crystallography and ultrafast laser spectroscopy., Doust AB, Marai CN, Harrop SJ, Wilk KE, Curmi PM, Scholes GD, J Mol Biol. 2004 Nov 12;344(1):135-53. PMID:15504407

Evolution of a light-harvesting protein by addition of new subunits and rearrangement of conserved elements: crystal structure of a cryptophyte phycoerythrin at 1.63-A resolution., Wilk KE, Harrop SJ, Jankova L, Edler D, Keenan G, Sharples F, Hiller RG, Curmi PM, Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8901-6. PMID:10430868

Phycobilins of cryptophycean algae. Occurrence of dihydrobiliverdin and mesobiliverdin in cryptomonad biliproteins., Wedemayer GJ, Kidd DG, Wemmer DE, Glazer AN, J Biol Chem. 1992 Apr 15;267(11):7315-31. PMID:1559975

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