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1qe3

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[[Image:1qe3.jpg|left|200px]]
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{{Seed}}
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[[Image:1qe3.png|left|200px]]
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{{STRUCTURE_1qe3| PDB=1qe3 | SCENE= }}
{{STRUCTURE_1qe3| PDB=1qe3 | SCENE= }}
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'''PNB ESTERASE'''
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===PNB ESTERASE===
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==Overview==
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Two directed evolution experiments on p-nitrobenzyl esterase yielded one enzyme with a 100-fold increased activity in aqueous-organic solvents and another with a 17 degrees C increase in thermostability. Structures of the wild type and its organophilic and thermophilic counterparts are presented at resolutions of 1.5 A, 1.6 A, and 2.0 A, respectively. These structures identify groups of interacting mutations and demonstrate how directed evolution can traverse complex fitness landscapes. Early-generation mutations stabilize flexible loops not visible in the wild-type structure and set the stage for further beneficial mutations in later generations. The mutations exert their influence on the esterase structure over large distances, in a manner that would be difficult to predict. The loops with the largest structural changes generally are not the sites of mutations. Similarly, none of the seven amino acid substitutions in the organophile are in the active site, even though the enzyme experiences significant changes in the organization of this site. In addition to reduction of surface loop flexibility, thermostability in the evolved esterase results from altered core packing, helix stabilization, and the acquisition of surface salt bridges, in agreement with other comparative studies of mesophilic and thermophilic enzymes. Crystallographic analysis of the wild type and its evolved counterparts reveals networks of mutations that collectively reorganize the active site. Interestingly, the changes that led to diversity within the alpha/beta hydrolase enzyme family and the reorganization seen in this study result from main-chain movements.
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(as it appears on PubMed at http://www.pubmed.gov), where 10535917 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10535917}}
==About this Structure==
==About this Structure==
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[[Category: Stevens, R.]]
[[Category: Stevens, R.]]
[[Category: Alpha-beta hydrolase directed evolution]]
[[Category: Alpha-beta hydrolase directed evolution]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:10:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:14:14 2008''

Revision as of 13:14, 28 July 2008

Image:1qe3.png

Template:STRUCTURE 1qe3

PNB ESTERASE

Template:ABSTRACT PUBMED 10535917

About this Structure

1QE3 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

A structural view of evolutionary divergence., Spiller B, Gershenson A, Arnold FH, Stevens RC, Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12305-10. PMID:10535917

Page seeded by OCA on Mon Jul 28 16:14:14 2008

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