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1v3h

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[[Image:1v3h.jpg|left|200px]]
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{{STRUCTURE_1v3h| PDB=1v3h | SCENE= }}
{{STRUCTURE_1v3h| PDB=1v3h | SCENE= }}
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'''The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase'''
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===The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase===
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==Overview==
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It has previously been suggested that the glutamic acid residues Glu186 and Glu380 of soybean beta-amylase play critical roles as a general acid and a general base catalyst, respectively. In order to confirm the roles of Glu186 and Glu380, each residue was mutated to a glutamine residue and the crystal structures of the substrate (E186Q/maltopentaose) and product (E380Q/maltose) complexes were determined at resolutions of 1.6 Angstrom and 1.9 Angstrom, respectively. Both mutant enzymes exhibited 16,000- and 37,000-fold decreased activity relative to that of the wild-type enzyme. The crystal structure of the E186Q/maltopentaose complex revealed an unambiguous five-glucose unit at subsites -2 to +3. Two maltose molecules bind on subsites -2 to -1 and +2 to +3 in the E380Q/maltose complex, whereas they bind in tandem to -2 to -1 and +1 to +2 in the wild-type/maltose complex. The conformation of the glucose residue at subsite -1 was identified as a stable (4)C(1) alpha-anomer in the E380Q/maltose complex, whereas a distorted ring conformation was observed in the wild-type/maltose complex. The side-chain movement of Gln380 to the position of a putative attacking water molecule seen in the wild-type enzyme caused the inactivation of the E380Q mutant and an altered binding pattern of maltose molecules. These results confirm the critical roles played by Glu186 in the donation of a proton to the glycosidic oxygen of the substrate, and by Glu380 in the activation of an attacking water molecule. The observed difference between the backbones of E186Q/maltopentaose and E380Q/maltose in terms of Thr342 suggests that the side-chain of Thr342 may stabilize the deprotonated form of Glu186 after the cleavage of the glycosidic bond.
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{{ABSTRACT_PUBMED_15178253}}
==About this Structure==
==About this Structure==
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[[Category: Mikami, B.]]
[[Category: Mikami, B.]]
[[Category: Utsumi, S.]]
[[Category: Utsumi, S.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:01:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:32:48 2008''

Revision as of 13:32, 28 July 2008

Image:1v3h.png

Template:STRUCTURE 1v3h

The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase

Template:ABSTRACT PUBMED 15178253

About this Structure

1V3H is a Single protein structure of sequence from Glycine max. Full crystallographic information is available from OCA.

Reference

The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase., Kang YN, Adachi M, Utsumi S, Mikami B, J Mol Biol. 2004 Jun 18;339(5):1129-40. PMID:15178253

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