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| | {{STRUCTURE_1v3h| PDB=1v3h | SCENE= }} | | {{STRUCTURE_1v3h| PDB=1v3h | SCENE= }} |
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| - | '''The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase'''
| + | ===The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase=== |
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| - | ==Overview==
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| - | It has previously been suggested that the glutamic acid residues Glu186 and Glu380 of soybean beta-amylase play critical roles as a general acid and a general base catalyst, respectively. In order to confirm the roles of Glu186 and Glu380, each residue was mutated to a glutamine residue and the crystal structures of the substrate (E186Q/maltopentaose) and product (E380Q/maltose) complexes were determined at resolutions of 1.6 Angstrom and 1.9 Angstrom, respectively. Both mutant enzymes exhibited 16,000- and 37,000-fold decreased activity relative to that of the wild-type enzyme. The crystal structure of the E186Q/maltopentaose complex revealed an unambiguous five-glucose unit at subsites -2 to +3. Two maltose molecules bind on subsites -2 to -1 and +2 to +3 in the E380Q/maltose complex, whereas they bind in tandem to -2 to -1 and +1 to +2 in the wild-type/maltose complex. The conformation of the glucose residue at subsite -1 was identified as a stable (4)C(1) alpha-anomer in the E380Q/maltose complex, whereas a distorted ring conformation was observed in the wild-type/maltose complex. The side-chain movement of Gln380 to the position of a putative attacking water molecule seen in the wild-type enzyme caused the inactivation of the E380Q mutant and an altered binding pattern of maltose molecules. These results confirm the critical roles played by Glu186 in the donation of a proton to the glycosidic oxygen of the substrate, and by Glu380 in the activation of an attacking water molecule. The observed difference between the backbones of E186Q/maltopentaose and E380Q/maltose in terms of Thr342 suggests that the side-chain of Thr342 may stabilize the deprotonated form of Glu186 after the cleavage of the glycosidic bond.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15178253}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15178253 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15178253}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Mikami, B.]] | | [[Category: Mikami, B.]] |
| | [[Category: Utsumi, S.]] | | [[Category: Utsumi, S.]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:01:54 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:32:48 2008'' |
Revision as of 13:32, 28 July 2008
Template:STRUCTURE 1v3h
The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase
Template:ABSTRACT PUBMED 15178253
About this Structure
1V3H is a Single protein structure of sequence from Glycine max. Full crystallographic information is available from OCA.
Reference
The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase., Kang YN, Adachi M, Utsumi S, Mikami B, J Mol Biol. 2004 Jun 18;339(5):1129-40. PMID:15178253
Page seeded by OCA on Mon Jul 28 16:32:48 2008
