This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2j6r

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2j6r.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2j6r.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2j6r| PDB=2j6r | SCENE= }}
{{STRUCTURE_2j6r| PDB=2j6r | SCENE= }}
-
'''FAEG FROM F4AC ETEC STRAIN GIS26, PRODUCED IN TOBACCO PLANT CHLOROPLAST'''
+
===FAEG FROM F4AC ETEC STRAIN GIS26, PRODUCED IN TOBACCO PLANT CHLOROPLAST===
-
==Overview==
+
<!--
-
F4 fimbriae encoded by the fae operon are the major colonization factors associated with porcine neonatal and postweaning diarrhoea caused by enterotoxigenic Escherichia coli (ETEC). Via the chaperone/usher pathway, the F4 fimbriae are assembled as long polymers of the major subunit FaeG, which also possesses the adhesive properties of the fimbriae. Intrinsically, the incomplete fold of fimbrial subunits renders them unstable and susceptible to aggregation and/or proteolytic degradation in the absence of a specific periplasmic chaperone. In order to test the possibility of producing FaeG in plants, FaeG expression was studied in transgenic tobacco plants. FaeG was directed to different subcellular compartments by specific targeting signals. Targeting of FaeG to the chloroplast results in much higher yields than FaeG targeting to the endoplasmic reticulum or the apoplast. Two chloroplast-targeted FaeG variants were purified from tobacco plants and crystallized. The crystal structures show that chloroplasts circumvent the absence of the fimbrial assembly machinery by assembling FaeG into strand-swapped dimers. Furthermore, the structures reveal how FaeG combines the structural requirements of a major fimbrial subunit with its adhesive role by grafting an additional domain on its Ig-like core.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17368480}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17368480 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17368480}}
==About this Structure==
==About this Structure==
Line 39: Line 43:
[[Category: Plasmid]]
[[Category: Plasmid]]
[[Category: Strand swapping]]
[[Category: Strand swapping]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:25:35 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:38:54 2008''

Revision as of 13:38, 28 July 2008

Image:2j6r.png

Template:STRUCTURE 2j6r

FAEG FROM F4AC ETEC STRAIN GIS26, PRODUCED IN TOBACCO PLANT CHLOROPLAST

Template:ABSTRACT PUBMED 17368480

About this Structure

2J6R is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Chloroplasts assemble the major subunit FaeG of Escherichia coli F4 (K88) fimbriae to strand-swapped dimers., Van Molle I, Joensuu JJ, Buts L, Panjikar S, Kotiaho M, Bouckaert J, Wyns L, Niklander-Teeri V, De Greve H, J Mol Biol. 2007 May 4;368(3):791-9. Epub 2007 Feb 22. PMID:17368480

Page seeded by OCA on Mon Jul 28 16:38:54 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j6r"
Personal tools