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2aty

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[[Image:2aty.gif|left|200px]]
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{{STRUCTURE_2aty| PDB=2aty | SCENE= }}
{{STRUCTURE_2aty| PDB=2aty | SCENE= }}
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'''Complement receptor chimaeric conjugate CR2-Ig'''
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===Complement receptor chimaeric conjugate CR2-Ig===
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==Overview==
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Complement receptor 2 (CR2; CD21) is a membrane-bound regulator of complement activation, being comprised of 15 or 16 short complement repeat (SCR) domains. A recombinant glycosylated human CR2 SCR 1-2 domain pair was engineered with the Fc fragment of a mouse IgG1 antibody to create a chimaera CR2-Ig containing the major ligand binding domains. Such a chimaera has therapeutic potential as a complement inhibitor or immune modulator. X-ray and neutron scattering and analytical ultracentrifugation identified its domain structure in solution, and provided a comparison with controversial folded-back crystal structures for deglycosylated CR2 SCR 1-2. The radius of gyration R(G) of CR2-Ig was determined to be 5.39(+/-0.14) nm and 5.29(+/-0.01) nm by X-ray and neutron scattering, respectively. The maximum dimension of CR2-Ig was determined to be 17 nm. The molecular mass of CR2-Ig ranged between 101,000 Da and 107,000 Da as determined by neutron scattering and sedimentation equilibrium, in good agreement with the sequence-derived value of 106,600 Da. Sedimentation velocity gave a sedimentation coefficient of 4.49(+/-0.11) S. Stereochemically complete models for CR2-Ig were constructed from crystal structures for the CR2 SCR 1-2 and mouse IgG1 Fc fragments. The two SCR domains and the Fc fragment were joined by randomised conformational peptides. The analysis of 35,000 possible CR2-Ig models showed that only those models in which the two SCR domains were arranged in an open V-shape in random orientations about the Fc fragment accounted for the scattering and sedimentation data. It was not possible to define one single conformational family of Fab-like fragment relative to the Fc fragment. This flexibility is attributed to the relatively long linker sequence and the absence of the antibody light chain from CR2-Ig. The modelling also confirmed that the structure of CR2 SCR 1-2 is more extended in solution than in its crystal structure.
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(as it appears on PubMed at http://www.pubmed.gov), where 16375923 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16375923}}
==About this Structure==
==About this Structure==
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[[Category: Complement]]
[[Category: Complement]]
[[Category: Immunoglobulin fold]]
[[Category: Immunoglobulin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:28:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:06:22 2008''

Revision as of 14:06, 28 July 2008

Image:2aty.png

Template:STRUCTURE 2aty

Complement receptor chimaeric conjugate CR2-Ig

Template:ABSTRACT PUBMED 16375923

About this Structure

2ATY is a Single protein structure of sequence from Homo sapiens, mus musculus. Full crystallographic information is available from OCA.

Reference

Extended flexible linker structures in the complement chimaeric conjugate CR2-Ig by scattering, analytical ultracentrifugation and constrained modelling: implications for function and therapy., Gilbert HE, Aslam M, Guthridge JM, Holers VM, Perkins SJ, J Mol Biol. 2006 Feb 17;356(2):397-412. Epub 2005 Dec 5. PMID:16375923

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