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1qaz

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{{STRUCTURE_1qaz| PDB=1qaz | SCENE= }}
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'''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION'''
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===CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION===
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==Overview==
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The three-dimensional structure of alginate lyase A1-III (ALYIII) from a Sphingomonas species A1 was determined by X-ray crystallography. The enzyme was crystallized by the hanging-drop vapour-diffusion method in the presence of 49% ammonium sulfate at 20 degrees C. The crystals are monoclinic and belong to the space group C2 with unit cell dimensions of a=49.18 A, b=93.08 A, c=82.10 A and beta=104.12 degrees. There was one molecule of alginate lyase in the asymmetric unit of the crystal. The diffraction data up to 1. 71 A were collected with Rsymof 5.0%. The crystal structure of ALYIII was solved by the multiple isomorphous replacement method and refined at 1.78 A resolution using X-PLOR with a final R -factor of 18.0% for 10.0 to 1.78 A resolution data. The refined model of ALYIII contained 351 amino acid residues, 299 water molecules and two sulfate ions. The three-dimensional structure of ALYIII was abundant in helices and had a deep tunnel-like cleft in a novel (alpha6/alpha5)-barrel structure, which was similar to the (alpha6/alpha6)-barrel found in glucoamylase and cellulase. This structure presented the possibility that alginate molecules might penetrate into the cleft to interact with the catalytic site of ALYIII.
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==About this Structure==
==About this Structure==
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[[Category: Crystal]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:10:45 2008''

Revision as of 14:10, 28 July 2008

Image:1qaz.png

Template:STRUCTURE 1qaz

CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION

Template:ABSTRACT PUBMED 10390348

About this Structure

1QAZ is a Single protein structure of sequence from Sphingomonas sp.. Full crystallographic information is available from OCA.

Reference

Crystal structure of alginate lyase A1-III from Sphingomonas species A1 at 1.78 A resolution., Yoon HJ, Mikami B, Hashimoto W, Murata K, J Mol Biol. 1999 Jul 9;290(2):505-14. PMID:10390348

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