1vbg

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{{STRUCTURE_1vbg| PDB=1vbg | SCENE= }}
{{STRUCTURE_1vbg| PDB=1vbg | SCENE= }}
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'''Pyruvate Phosphate Dikinase from Maize'''
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===Pyruvate Phosphate Dikinase from Maize===
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==Overview==
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Pyruvate phosphate dikinase (PPDK) reversibly catalyzes the conversion of ATP, phosphate, and pyruvate into AMP, pyrophosphate, and phosphoenolpyruvate (PEP), respectively. Since the nucleotide binding site (in the N-terminal domain) and the pyruvate/PEP binding site (in the C-terminal domain) are separated by approximately 45 A, it has been proposed that an intermediary domain, called the central domain, swivels between these remote domains to transfer the phosphate. However, no direct structural evidence for the swiveling central domain has been found. In this study, the crystal structures of maize PPDK with and without PEP have been determined at 2.3 A resolution. These structures revealed that the central domain is located near the pyruvate/PEP binding C-terminal domain, in contrast to the PPDK from Clostridium symbiosum, wherein the central domain is located near the nucleotide-binding N-terminal domain. Structural comparisons between the maize and C. symbiosum PPDKs demonstrated that the swiveling motion of the central domain consists of a rotation of at least 92 degrees and a translation of 0.5 A. By comparing the maize PPDK structures with and without PEP, we have elucidated the mode of binding of PEP to the C-terminal domain and the induced conformational changes in the central domain.
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The line below this paragraph, {{ABSTRACT_PUBMED_15667207}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15667207 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15667207}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structures of pyruvate phosphate dikinase from maize revealed an alternative conformation in the swiveling-domain motion., Nakanishi T, Nakatsu T, Matsuoka M, Sakata K, Kato H, Biochemistry. 2005 Feb 1;44(4):1136-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15667207 15667207]
Crystal structures of pyruvate phosphate dikinase from maize revealed an alternative conformation in the swiveling-domain motion., Nakanishi T, Nakatsu T, Matsuoka M, Sakata K, Kato H, Biochemistry. 2005 Feb 1;44(4):1136-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15667207 15667207]
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Purification, crystallization and preliminary X-ray diffraction studies on pyruvate phosphate dikinase from maize., Nakanishi T, Ohki Y, Oda J, Matsuoka M, Sakata K, Kato H, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):193-4. Epub 2003, Dec 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14684927 14684927]
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Primary structure of maize pyruvate, orthophosphate dikinase as deduced from cDNA sequence., Matsuoka M, Ozeki Y, Yamamoto N, Hirano H, Kano-Murakami Y, Tanaka Y, J Biol Chem. 1988 Aug 15;263(23):11080-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2841317 2841317]
[[Category: Pyruvate, phosphate dikinase]]
[[Category: Pyruvate, phosphate dikinase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:20:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:36:17 2008''

Revision as of 14:36, 28 July 2008

Image:1vbg.png

Template:STRUCTURE 1vbg

Pyruvate Phosphate Dikinase from Maize

Template:ABSTRACT PUBMED 15667207

About this Structure

1VBG is a Single protein structure of sequence from Zea mays. Full crystallographic information is available from OCA.

Reference

Crystal structures of pyruvate phosphate dikinase from maize revealed an alternative conformation in the swiveling-domain motion., Nakanishi T, Nakatsu T, Matsuoka M, Sakata K, Kato H, Biochemistry. 2005 Feb 1;44(4):1136-44. PMID:15667207

Purification, crystallization and preliminary X-ray diffraction studies on pyruvate phosphate dikinase from maize., Nakanishi T, Ohki Y, Oda J, Matsuoka M, Sakata K, Kato H, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):193-4. Epub 2003, Dec 18. PMID:14684927

Primary structure of maize pyruvate, orthophosphate dikinase as deduced from cDNA sequence., Matsuoka M, Ozeki Y, Yamamoto N, Hirano H, Kano-Murakami Y, Tanaka Y, J Biol Chem. 1988 Aug 15;263(23):11080-3. PMID:2841317

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