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2mrt

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[[Image:2mrt.jpg|left|200px]]
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{{Seed}}
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[[Image:2mrt.png|left|200px]]
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{{STRUCTURE_2mrt| PDB=2mrt | SCENE= }}
{{STRUCTURE_2mrt| PDB=2mrt | SCENE= }}
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'''CONFORMATION OF CD-7 METALLOTHIONEIN-2 FROM RAT LIVER IN AQUEOUS SOLUTION DETERMINED BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY'''
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===CONFORMATION OF CD-7 METALLOTHIONEIN-2 FROM RAT LIVER IN AQUEOUS SOLUTION DETERMINED BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY===
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==Overview==
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The three-dimensional structure of [Cd7]-metallothionein-2 from rat liver was determined in aqueous solution, using nuclear magnetic resonance spectrometry and distance geometry calculations. The experimental data provided proton-proton distance constraints from measurements of nuclear Overhauser effects, constraints on the geometry of the metal-cysteine clusters determined by heteronuclear correlation spectroscopy, and dihedral angle constraints derived from both coupling constants and nuclear Overhauser effects. The structure calculations were performed with the program DISMAN. As in previous studies with rabbit liver metallothionein-2a, the structure calculations were performed separately for the alpha and beta-domains containing the 4 and 3-metal clusters, respectively, since no interdomain constraints were found. For both domains, the global polypeptide fold, the location of polypeptide secondary structure elements, the architecture of the metal-sulfur cluster and the local chirality of the metal co-ordination are very similar to the solution structure of rabbit metallothionein-2a, but show considerable difference relative to the crystal structure of rat metallothionein-2.
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The line below this paragraph, {{ABSTRACT_PUBMED_3184190}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 3184190 is the PubMed ID number.
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{{ABSTRACT_PUBMED_3184190}}
==About this Structure==
==About this Structure==
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2MRT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MRT OCA].
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2MRT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MRT OCA].
==Reference==
==Reference==
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[[Category: Wuthrich, K.]]
[[Category: Wuthrich, K.]]
[[Category: Metallothionein]]
[[Category: Metallothionein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:34:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:38:22 2008''

Revision as of 14:38, 28 July 2008

Image:2mrt.png

Template:STRUCTURE 2mrt

CONFORMATION OF CD-7 METALLOTHIONEIN-2 FROM RAT LIVER IN AQUEOUS SOLUTION DETERMINED BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Template:ABSTRACT PUBMED 3184190

About this Structure

2MRT is a Single protein structure of sequence from Rattus rattus. Full experimental information is available from OCA.

Reference

Conformation of [Cd7]-metallothionein-2 from rat liver in aqueous solution determined by nuclear magnetic resonance spectroscopy., Schultze P, Worgotter E, Braun W, Wagner G, Vasak M, Kagi JH, Wuthrich K, J Mol Biol. 1988 Sep 5;203(1):251-68. PMID:3184190

Page seeded by OCA on Mon Jul 28 17:38:22 2008

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