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2p1x

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[[Image:2p1x.jpg|left|200px]]
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{{Seed}}
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[[Image:2p1x.png|left|200px]]
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{{STRUCTURE_2p1x| PDB=2p1x | SCENE= }}
{{STRUCTURE_2p1x| PDB=2p1x | SCENE= }}
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'''Crystal structure analysis of the complex between CyaY and Eu(III)'''
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===Crystal structure analysis of the complex between CyaY and Eu(III)===
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==Overview==
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Deficiency of the small mitochondrial protein frataxin causes Friedreich's ataxia, a severe neurodegenerative pathology. Frataxin, which has been highly conserved throughout evolution, is thought to be involved in, among other processes, Fe-S cluster formation. Independent evidence shows that it binds iron directly, although with very distinct features and low affinity. Here, we have carried out an extensive study of the binding properties of CyaY, the bacterial ortholog of frataxin, to different divalent and trivalent cations, using NMR and X-ray crystallography. We demonstrate that the protein has low cation specificity and contains multiple binding sites able to chelate divalent and trivalent metals with low affinity. Binding does not involve cavities or pockets, but exposed glutamates and aspartates, which are residues that are unusual for iron chelation when not assisted by histidines and/or cysteines. We have related how such an ability to bind cations on a relatively large area through an electrostatic mechanism could be a valuable asset for protein function.
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The line below this paragraph, {{ABSTRACT_PUBMED_17651435}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17651435 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17651435}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Understanding the binding properties of an unusual metal-binding protein--a study of bacterial frataxin., Pastore C, Franzese M, Sica F, Temussi P, Pastore A, FEBS J. 2007 Aug;274(16):4199-210. Epub 2007 Jul 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17651435 17651435]
Understanding the binding properties of an unusual metal-binding protein--a study of bacterial frataxin., Pastore C, Franzese M, Sica F, Temussi P, Pastore A, FEBS J. 2007 Aug;274(16):4199-210. Epub 2007 Jul 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17651435 17651435]
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Solution structure of the bacterial frataxin ortholog, CyaY: mapping the iron binding sites., Nair M, Adinolfi S, Pastore C, Kelly G, Temussi P, Pastore A, Structure. 2004 Nov;12(11):2037-48. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15530368 15530368]
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Crystal structure of Escherichia coli CyaY protein reveals a previously unidentified fold for the evolutionarily conserved frataxin family., Cho SJ, Lee MG, Yang JK, Lee JY, Song HK, Suh SW, Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8932-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10908679 10908679]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Friedreich's ataxia iron binding,iron-sulfur cluster assembly]]
[[Category: Friedreich's ataxia iron binding,iron-sulfur cluster assembly]]
[[Category: Unknown function]]
[[Category: Unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:10:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:48:08 2008''

Revision as of 14:48, 28 July 2008

Image:2p1x.png

Template:STRUCTURE 2p1x

Crystal structure analysis of the complex between CyaY and Eu(III)

Template:ABSTRACT PUBMED 17651435

About this Structure

2P1X is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Understanding the binding properties of an unusual metal-binding protein--a study of bacterial frataxin., Pastore C, Franzese M, Sica F, Temussi P, Pastore A, FEBS J. 2007 Aug;274(16):4199-210. Epub 2007 Jul 25. PMID:17651435

Solution structure of the bacterial frataxin ortholog, CyaY: mapping the iron binding sites., Nair M, Adinolfi S, Pastore C, Kelly G, Temussi P, Pastore A, Structure. 2004 Nov;12(11):2037-48. PMID:15530368

Crystal structure of Escherichia coli CyaY protein reveals a previously unidentified fold for the evolutionarily conserved frataxin family., Cho SJ, Lee MG, Yang JK, Lee JY, Song HK, Suh SW, Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8932-7. PMID:10908679

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