3bck

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{{STRUCTURE_3bck| PDB=3bck | SCENE= }}
{{STRUCTURE_3bck| PDB=3bck | SCENE= }}
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'''Crystal Structure of Staphylococcus aureus DsbA T153V'''
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===Crystal Structure of Staphylococcus aureus DsbA T153V===
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==Overview==
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In Gram-negative bacteria, the introduction of disulfide bonds into folding proteins occurs in the periplasm and is catalyzed by donation of an energetically unstable disulfide from DsbA, which is subsequently re-oxidized through interaction with DsbB. Gram-positive bacteria lack a classic periplasm but nonetheless encode Dsb-like proteins. Staphylococcus aureus encodes just one Dsb protein, a DsbA, and no DsbB. Here we report the crystal structure of S. aureus DsbA (SaDsbA), which incorporates a thioredoxin fold with an inserted helical domain, like its Escherichia coli counterpart EcDsbA, but it lacks the characteristic hydrophobic patch and has a truncated binding groove near the active site. These findings suggest that SaDsbA has a different substrate specificity than EcDsbA. Thermodynamic studies indicate that the oxidized and reduced forms of SaDsbA are energetically equivalent, in contrast to the energetically unstable disulfide form of EcDsbA. Further, the partial complementation of EcDsbA by SaDsbA is independent of EcDsbB and biochemical assays show that SaDsbA does not interact with EcDsbB. The identical stabilities of oxidized and reduced SaDsbA may facilitate direct re-oxidation of the protein by extracellular oxidants, without the need for DsbB.
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{{ABSTRACT_PUBMED_18077463}}
==About this Structure==
==About this Structure==
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[[Category: Redox protein]]
[[Category: Redox protein]]
[[Category: Thiol-disulfide oxidoreductase]]
[[Category: Thiol-disulfide oxidoreductase]]
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Revision as of 16:01, 28 July 2008

Image:3bck.png

Template:STRUCTURE 3bck

Crystal Structure of Staphylococcus aureus DsbA T153V

Template:ABSTRACT PUBMED 18077463

About this Structure

3BCK is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

Staphylococcus aureus DsbA Does Not Have a Destabilizing Disulfide: A NEW PARADIGM FOR BACTERIAL OXIDATIVE FOLDING., Heras B, Kurz M, Jarrott R, Shouldice SR, Frei P, Robin G, Cemazar M, Thony-Meyer L, Glockshuber R, Martin JL, J Biol Chem. 2008 Feb 15;283(7):4261-71. Epub 2007 Dec 12. PMID:18077463

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