This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2dwu

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2dwu.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2dwu.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2dwu| PDB=2dwu | SCENE= }}
{{STRUCTURE_2dwu| PDB=2dwu | SCENE= }}
-
'''Crystal Structure of Glutamate Racemase Isoform RacE1 from Bacillus anthracis'''
+
===Crystal Structure of Glutamate Racemase Isoform RacE1 from Bacillus anthracis===
-
==Overview==
+
<!--
-
Glutamate racemase (RacE) is responsible for converting l-glutamate to d-glutamate, which is an essential component of peptidoglycan biosynthesis, and the primary constituent of the poly-gamma-d-glutamate capsule of the pathogen Bacillus anthracis. RacE enzymes are essential for bacterial growth and lack a human homolog, making them attractive targets for the design and development of antibacterial therapeutics. We have cloned, expressed and purified the two glutamate racemase isozymes, RacE1 and RacE2, from the B. anthracis genome. Through a series of steady-state kinetic studies, and based upon the ability of both RacE1 and RacE2 to catalyze the rapid formation of d-glutamate, we have determined that RacE1 and RacE2 are bona fide isozymes. The X-ray structures of B. anthracis RacE1 and RacE2, in complex with d-glutamate, were determined to resolutions of 1.75 A and 2.0 A. Both enzymes are dimers with monomers arranged in a "tail-to-tail" orientation, similar to the B. subtilis RacE structure, but differing substantially from the Aquifex pyrophilus RacE structure. The differences in quaternary structures produce differences in the active sites of racemases among the various species, which has important implications for structure-based, inhibitor design efforts within this class of enzymes. We found a Val to Ala variance at the entrance of the active site between RacE1 and RacE2, which results in the active site entrance being less sterically hindered for RacE1. Using a series of inhibitors, we show that this variance results in differences in the inhibitory activity against the two isozymes and suggest a strategy for structure-based inhibitor design to obtain broad-spectrum inhibitors for glutamate racemases.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17610893}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17610893 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17610893}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Santarsiero, B D.]]
[[Category: Santarsiero, B D.]]
[[Category: Racemase]]
[[Category: Racemase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:32:20 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:01:39 2008''

Revision as of 16:01, 28 July 2008

Image:2dwu.png

Template:STRUCTURE 2dwu

Crystal Structure of Glutamate Racemase Isoform RacE1 from Bacillus anthracis

Template:ABSTRACT PUBMED 17610893

About this Structure

2DWU is a Single protein structure of sequence from Bacillus anthracis. Full crystallographic information is available from OCA.

Reference

Structural and functional analysis of two glutamate racemase isozymes from Bacillus anthracis and implications for inhibitor design., May M, Mehboob S, Mulhearn DC, Wang Z, Yu H, Thatcher GR, Santarsiero BD, Johnson ME, Mesecar AD, J Mol Biol. 2007 Aug 31;371(5):1219-37. Epub 2007 Jun 4. PMID:17610893

Page seeded by OCA on Mon Jul 28 19:01:39 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2dwu"
Personal tools