1ryr

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{{STRUCTURE_1ryr| PDB=1ryr | SCENE= }}
{{STRUCTURE_1ryr| PDB=1ryr | SCENE= }}
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'''REPLICATION OF A CIS-SYN THYMINE DIMER AT ATOMIC RESOLUTION'''
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===REPLICATION OF A CIS-SYN THYMINE DIMER AT ATOMIC RESOLUTION===
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==Overview==
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Ultraviolet light damages DNA by catalysing covalent bond formation between adjacent pyrimidines, generating cis-syn cyclobutane pyrimidine dimers (CPDs) as the most common lesion. CPDs block DNA replication by high-fidelity DNA polymerases, but they can be efficiently bypassed by the Y-family DNA polymerase pol eta. Mutations in POLH encoding pol eta are implicated in nearly 20% of xeroderma pigmentosum, a human disease characterized by extreme sensitivity to sunlight and predisposition to skin cancer. Here we have determined two crystal structures of Dpo4, an archaeal pol eta homologue, complexed with CPD-containing DNA, where the 3' and 5' thymine of the CPD separately serves as a templating base. The 3' thymine of the CPD forms a Watson-Crick base pair with the incoming dideoxyATP, but the 5' thymine forms a Hoogsteen base pair with the dideoxyATP in syn conformation. Dpo4 retains a similar tertiary structure, but each unusual DNA structure is individually fitted into the active site for catalysis. A model of the pol eta-CPD complex built from the crystal structures of Saccharomyces cerevisiae apo-pol eta and the Dpo4-CPD complex suggests unique features that allow pol eta to efficiently bypass CPDs.
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(as it appears on PubMed at http://www.pubmed.gov), where 12904819 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12904819}}
==About this Structure==
==About this Structure==
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[[Category: Polymerase]]
[[Category: Polymerase]]
[[Category: Tt-dimer]]
[[Category: Tt-dimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:04:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:20:52 2008''

Revision as of 16:20, 28 July 2008

Image:1ryr.png

Template:STRUCTURE 1ryr

REPLICATION OF A CIS-SYN THYMINE DIMER AT ATOMIC RESOLUTION

Template:ABSTRACT PUBMED 12904819

About this Structure

1RYR is a Single protein structure of sequence from Sulfolobus solfataricus. This structure supersedes the now removed PDB entry 1pm0. Full crystallographic information is available from OCA.

Reference

Replication of a cis-syn thymine dimer at atomic resolution., Ling H, Boudsocq F, Plosky BS, Woodgate R, Yang W, Nature. 2003 Aug 28;424(6952):1083-7. Epub 2003 Aug 6. PMID:12904819

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