1xxa

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{{STRUCTURE_1xxa| PDB=1xxa | SCENE= }}
{{STRUCTURE_1xxa| PDB=1xxa | SCENE= }}
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'''C-TERMINAL DOMAIN OF ESCHERICHIA COLI ARGININE REPRESSOR/ L-ARGININE COMPLEX; PB DERIVATIVE'''
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===C-TERMINAL DOMAIN OF ESCHERICHIA COLI ARGININE REPRESSOR/ L-ARGININE COMPLEX; PB DERIVATIVE===
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==Overview==
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The structure of the oligomerization and L-arginine binding domain of the Escherichia coli arginine repressor (ArgR) has been determined using X-ray diffraction methods at 2.2 A resolution with bound arginine and at 2.8 A in the unliganded form. The oligomeric core is a 3-fold rotationally symmetric hexamer formed from six identical subunits corresponding to the 77 C-terminal residues (80 to 156) of ArgR. Each subunit has an alpha/beta fold containing a four-stranded antiparallel beta-sheet and two antiparallel alpha-helices. The hexamer is formed from two trimers, each with tightly packed hydrophobic cores. In the absence of arginine, the trimers stack back-to-back through a dyad-symmetric, sparsely packed hydrophobic interface. Six molecules of arginine bind at the trimer-trimer interface, each making ten hydrogen bonds to the protein including a direct ion pair that crosslinks the two protein trimers. Solution experiments with wild-type ArgR and oligomerization domain indicate that the hexameric form is greatly stabilized upon arginine binding. The crystal structures and solution experiments together suggest possible mechanisms of how arginine activates ArgR to bind to its DNA targets and provides a stereochemical basis for interpreting the results of mutagenesis and biochemical experiments with ArgR.
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{{ABSTRACT_PUBMED_8594204}}
==About this Structure==
==About this Structure==
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[[Category: Maas, W K.]]
[[Category: Maas, W K.]]
[[Category: Sigler, P B.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:36:23 2008''

Revision as of 16:36, 28 July 2008

Image:1xxa.png

Template:STRUCTURE 1xxa

C-TERMINAL DOMAIN OF ESCHERICHIA COLI ARGININE REPRESSOR/ L-ARGININE COMPLEX; PB DERIVATIVE

Template:ABSTRACT PUBMED 8594204

About this Structure

1XXA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of the oligomerization and L-arginine binding domain of the arginine repressor of Escherichia coli., Van Duyne GD, Ghosh G, Maas WK, Sigler PB, J Mol Biol. 1996 Feb 23;256(2):377-91. PMID:8594204

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