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1sxt

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{{STRUCTURE_1sxt| PDB=1sxt | SCENE= }}
{{STRUCTURE_1sxt| PDB=1sxt | SCENE= }}
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'''STAPHYLOCOCCAL ENTEROTOXIN TYPE A (SEA) CO-CRYSTALLISED WITH ZINC'''
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===STAPHYLOCOCCAL ENTEROTOXIN TYPE A (SEA) CO-CRYSTALLISED WITH ZINC===
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==Overview==
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Superantigens form complexes with major histocompatibility complex (MHC) class II molecules and T-cell receptors resulting in extremely strong immunostimulatory properties. Staphylococcus aureus enterotoxin A (SEA) belongs to a subgroup of the staphylococcal superantigens that utilizes Zn2+ in the high affinity interaction with MHC class II molecules. A high affinity metal binding site was described previously in SEA co-crystallized with Cd2+ in which the metal ion was octahedrally co-ordinated, involving the N-terminal serine. We have now co-crystallized SEA with its native co-factor Zn2+ and determined its crystal structure at 2.7 A resolution. As expected for a Zn2+ ion, the co-ordination was found to be tetrahedral. Three of the ligands are located on the SEA surface on a C-terminal domain beta-sheet, while the fourth varies with the conditions. Further analysis of the zinc binding event was performed using titration microcalorimetry, which showed that SEA binds Zn2+ with an affinity of KD = 0.3 microM in an entropy driven process. The differential Zn2+ co-ordination observed here has implications for the mechanism of the SEA-MHC class II interaction.
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(as it appears on PubMed at http://www.pubmed.gov), where 8943278 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8943278}}
==About this Structure==
==About this Structure==
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[[Category: Superantigen]]
[[Category: Superantigen]]
[[Category: Toxin]]
[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:15:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:03:00 2008''

Revision as of 18:03, 28 July 2008

Image:1sxt.png

Template:STRUCTURE 1sxt

STAPHYLOCOCCAL ENTEROTOXIN TYPE A (SEA) CO-CRYSTALLISED WITH ZINC

Template:ABSTRACT PUBMED 8943278

About this Structure

1SXT is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

The Co-crystal structure of staphylococcal enterotoxin type A with Zn2+ at 2.7 A resolution. Implications for major histocompatibility complex class II binding., Sundstrom M, Hallen D, Svensson A, Schad E, Dohlsten M, Abrahmsen L, J Biol Chem. 1996 Dec 13;271(50):32212-6. PMID:8943278

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