1vfe

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{{STRUCTURE_1vfe| PDB=1vfe | SCENE= }}
{{STRUCTURE_1vfe| PDB=1vfe | SCENE= }}
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'''HUMAN LACTOFERRIN, N-TERMINAL LOBE MUTANT WITH ARG 121 REPLACED BY SER (R121S)'''
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===HUMAN LACTOFERRIN, N-TERMINAL LOBE MUTANT WITH ARG 121 REPLACED BY SER (R121S)===
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==Overview==
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A conserved arginine residue helps to form the synergistic anion binding site in transferrins. To probe the importance of this residue for anion binding and iron binding, Arg 121 has been mutated to Ser and Glu in N-terminal half-molecule of human lactoferrin. The two mutants, R121S and R121E, have been expressed, purified, and crystallized. Their three-dimensional structures have been determined by X-ray diffraction at 2.3 and 2.5 A resolution, respectively. The structures were determined by molecular replacement and were refined by restrained least squares methods to final R values of 0.185 and 0.204. Both mutants still bind iron but with decreased stability. The crystal structures show that destabilization of iron binding probably results from disruption of the anion binding site; mutation of Arg 121 removes one wall of the anion binding pocket and causes the synergistic carbonate ion to be displaced 0.5 A from its position in the wild-type protein. In the process it becomes partially detached from the helix N-terminus that forms the rest of the anion binding site.
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{{ABSTRACT_PUBMED_8931543}}
==About this Structure==
==About this Structure==
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[[Category: Recombinant half molecule]]
[[Category: Recombinant half molecule]]
[[Category: Transferrin]]
[[Category: Transferrin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:28:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 22:09:07 2008''

Revision as of 19:09, 28 July 2008

Image:1vfe.png

Template:STRUCTURE 1vfe

HUMAN LACTOFERRIN, N-TERMINAL LOBE MUTANT WITH ARG 121 REPLACED BY SER (R121S)

Template:ABSTRACT PUBMED 8931543

About this Structure

1VFE is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Mutation of arginine 121 in lactoferrin destabilizes iron binding by disruption of anion binding: crystal structures of R121S and R121E mutants., Faber HR, Baker CJ, Day CL, Tweedie JW, Baker EN, Biochemistry. 1996 Nov 19;35(46):14473-9. PMID:8931543

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