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1r62

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{{STRUCTURE_1r62| PDB=1r62 | SCENE= }}
{{STRUCTURE_1r62| PDB=1r62 | SCENE= }}
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'''Crystal structure of the C-terminal Domain of the Two-Component System Transmitter Protein NRII (NtrB)'''
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===Crystal structure of the C-terminal Domain of the Two-Component System Transmitter Protein NRII (NtrB)===
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==Overview==
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The kinase/phosphatase nitrogen regulator II (NRII, NtrB) is a member of the transmitter protein family of conserved two-component signal transduction systems. The kinase activity of NRII brings about the phosphorylation of the transcription factor nitrogen regulator I (NRI, NtrC), causing the activation of Ntr gene transcription. The phosphatase activity of NRII results in the inactivation of NRI-P. The activities of NRII are regulated by the signal transduction protein encoded by glnB, PII protein, which upon binding to NRII inhibits the kinase and activates the phosphatase activity. The C-terminal ATP-binding domain of NRII is required for both the kinase and phosphatase activities and contains the PII binding site. Here, we present the crystal structure of the C-terminal domain of a mutant form of NRII, NRII-Y302N, at 1.6 A resolution and compare this structure to the analogous domains of other two-component system transmitter proteins. While the C-terminal domain of NRII shares the general tertiary structure seen in CheA, PhoQ, and EnvZ transmitter proteins, it contains a distinct beta-hairpin projection that is absent in these related proteins. This projection is near the site of a well-characterized mutation that reduces the binding of PII and near other less-characterized mutations that affect the phosphatase activity of NRII. Sequence alignment suggests that the beta-hairpin projection is present in NRII proteins from various organisms, and absent in other transmitter proteins from Escherichia coliK-12. This unique structural element in the NRII C-terminal domain may play a role in binding PII or in intramolecular signal transduction.
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{{ABSTRACT_PUBMED_15157101}}
==About this Structure==
==About this Structure==
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[[Category: Pii]]
[[Category: Pii]]
[[Category: Two component system]]
[[Category: Two component system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:07:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 22:56:48 2008''

Revision as of 19:57, 28 July 2008

Image:1r62.png

Template:STRUCTURE 1r62

Crystal structure of the C-terminal Domain of the Two-Component System Transmitter Protein NRII (NtrB)

Template:ABSTRACT PUBMED 15157101

About this Structure

1R62 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of the C-terminal domain of the two-component system transmitter protein nitrogen regulator II (NRII; NtrB), regulator of nitrogen assimilation in Escherichia coli., Song Y, Peisach D, Pioszak AA, Xu Z, Ninfa AJ, Biochemistry. 2004 Jun 1;43(21):6670-8. PMID:15157101

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