1omv

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[[Image:1omv.jpg|left|200px]]
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{{Seed}}
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[[Image:1omv.png|left|200px]]
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{{STRUCTURE_1omv| PDB=1omv | SCENE= }}
{{STRUCTURE_1omv| PDB=1omv | SCENE= }}
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'''non-myristoylated bovine recoverin (E85Q mutant) with calcium bound to EF-hand 3'''
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===non-myristoylated bovine recoverin (E85Q mutant) with calcium bound to EF-hand 3===
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==Overview==
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Recoverin is a Ca2+-regulated signal transduction modulator found in vertebrate retina that has been shown to undergo dramatic conformational changes upon Ca2+ binding to its two functional EF-hand motifs. To elucidate the differential impact of the N-terminal myristoylation as well as occupation of the two Ca2+ binding sites on recoverin structure and function, we have investigated a non-myristoylated E85Q mutant exhibiting virtually no Ca2+ binding to EF-2. Crystal structures of the mutant protein as well as the non-myristoylated wild-type have been determined. Although the non-myristoylated E85Q mutant does not display any functional activity, its three-dimensional structure in the presence of Ca2+ resembles the myristoylated wild-type with two Ca2+ but is quite dissimilar from the myristoylated E85Q mutant. We conclude that the N-terminal myristoyl modification significantly stabilizes the conformation of the Ca2+-free protein (i.e. the T conformation) during the stepwise transition toward the fully Ca2+-occupied state. On the basis of these observations, a refined model for the role of the myristoyl group as an intrinsic allosteric modulator is proposed.
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(as it appears on PubMed at http://www.pubmed.gov), where 12686556 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12686556}}
==About this Structure==
==About this Structure==
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[[Category: Ef-hand]]
[[Category: Ef-hand]]
[[Category: Helix-loop-helix]]
[[Category: Helix-loop-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:02:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:02:24 2008''

Revision as of 20:02, 28 July 2008

Image:1omv.png

Template:STRUCTURE 1omv

non-myristoylated bovine recoverin (E85Q mutant) with calcium bound to EF-hand 3

Template:ABSTRACT PUBMED 12686556

About this Structure

1OMV is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Impact of N-terminal myristoylation on the Ca2+-dependent conformational transition in recoverin., Weiergraber OH, Senin II, Philippov PP, Granzin J, Koch KW, J Biol Chem. 2003 Jun 20;278(25):22972-9. Epub 2003 Apr 9. PMID:12686556

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