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2yva

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{{STRUCTURE_2yva| PDB=2yva | SCENE= }}
{{STRUCTURE_2yva| PDB=2yva | SCENE= }}
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'''Crystal structure of Escherichia coli DiaA'''
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===Crystal structure of Escherichia coli DiaA===
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==Overview==
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Escherichia coli DiaA is a DnaA-binding protein that is required for the timely initiation of chromosomal replication during the cell cycle. In this study, we determined the crystal structure of DiaA at 1.8 A resolution. DiaA forms a homotetramer consisting of a symmetrical pair of homodimers. Mutational analysis revealed that the DnaA-binding activity and formation of homotetramers are required for the stimulation of initiation by DiaA. DiaA tetramers can bind multiple DnaA molecules simultaneously. DiaA stimulated the assembly of multiple DnaA molecules on oriC, conformational changes in ATP-DnaA-specific initiation complexes, and unwinding of oriC duplex DNA. The mutant DiaA proteins are defective in these stimulations. DiaA associated also with ADP-DnaA, and stimulated the assembly of inactive ADP-DnaA-oriC complexes. Specific residues in the putative phosphosugar-binding motif of DiaA were required for the stimulation of initiation and formation of ATP-DnaA-specific-oriC complexes. Our data indicate that DiaA regulates initiation by a novel mechanism, in which DiaA tetramers most likely bind to multiple DnaA molecules and stimulate the assembly of specific ATP-DnaA-oriC complexes. These results suggest an essential role for DiaA in the promotion of replication initiation in a cell cycle coordinated manner.
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{{ABSTRACT_PUBMED_17699754}}
==About this Structure==
==About this Structure==
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[[Category: Rsgi]]
[[Category: Rsgi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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Revision as of 20:26, 28 July 2008

Image:2yva.png

Template:STRUCTURE 2yva

Crystal structure of Escherichia coli DiaA

Template:ABSTRACT PUBMED 17699754

About this Structure

2YVA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP DnaA-specific initiation complexes., Keyamura K, Fujikawa N, Ishida T, Ozaki S, Su'etsugu M, Fujimitsu K, Kagawa W, Yokoyama S, Kurumizaka H, Katayama T, Genes Dev. 2007 Aug 15;21(16):2083-99. PMID:17699754

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