This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2d17

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2d17.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2d17.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2d17| PDB=2d17 | SCENE= }}
{{STRUCTURE_2d17| PDB=2d17 | SCENE= }}
-
'''Solution RNA structure of stem-bulge-stem region of the HIV-1 dimerization initiation site'''
+
===Solution RNA structure of stem-bulge-stem region of the HIV-1 dimerization initiation site===
-
==Overview==
+
<!--
-
Dimer formation of HIV-1 genomic RNA through its dimerization initiation site (DIS) is crucial to maintaining infectivity. Two types of dimers, the initially generated kissing-loop dimer and the subsequent product of the extended-duplex dimer, are formed in the stem-bulge-stem region with a loop including a self-complementary sequence. NMR chemical shift analysis of a 39mer RNA corresponding to DIS, DIS39, in the kissing-loop and extended-duplex dimers revealed that the three dimensional structures of the stem-bulge-stem region are extremely similar between the two types of dimers. Therefore, we designed two shorter RNA molecules, loop25 and bulge34, corresponding to the loop-stem region and the stem-bulge-stem region of DIS39, respectively. Based upon the chemical shift analysis, the conformation of the loop region of loop25 is identical to that of DIS39 for each of the two types of dimers. The conformation of bulge34 was also found to be the same as that of the corresponding region of DIS39. Thus, we determined the solution structures of loop25 in each of the two types of dimers as well as that of bulge34. Finally, the solution structures of DIS39 in the kissing-loop and extended-duplex dimers were determined by combining the parts of the structures. The solution structures of the two types of dimers were similar to each other in general with a difference found only in the A16 residue. The elucidation of the structures of DIS39 is important to understanding the molecular mechanism of the conformational dynamics of viral RNA molecules.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16272570}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16272570 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16272570}}
==About this Structure==
==About this Structure==
-
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D17 OCA].
+
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D17 OCA].
==Reference==
==Reference==
Line 32: Line 36:
[[Category: Rna]]
[[Category: Rna]]
[[Category: Structure]]
[[Category: Structure]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:30:04 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:37:49 2008''

Revision as of 21:37, 28 July 2008

Image:2d17.png

Template:STRUCTURE 2d17

Solution RNA structure of stem-bulge-stem region of the HIV-1 dimerization initiation site

Template:ABSTRACT PUBMED 16272570

About this Structure

Full experimental information is available from OCA.

Reference

Solution RNA structures of the HIV-1 dimerization initiation site in the kissing-loop and extended-duplex dimers., Baba S, Takahashi K, Noguchi S, Takaku H, Koyanagi Y, Yamamoto N, Kawai G, J Biochem. 2005 Nov;138(5):583-92. PMID:16272570

Page seeded by OCA on Tue Jul 29 00:37:49 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2d17"
Personal tools