1p6c

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1p6c.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1p6c.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1p6c| PDB=1p6c | SCENE= }}
{{STRUCTURE_1p6c| PDB=1p6c | SCENE= }}
-
'''crystal structure of phosphotriesterase triple mutant H254G/H257W/L303T complexed with diisopropylmethylphosphonate'''
+
===crystal structure of phosphotriesterase triple mutant H254G/H257W/L303T complexed with diisopropylmethylphosphonate===
-
==Overview==
+
<!--
-
The bacterial phosphotriesterase has been utilized as a template for the evolution of improved enzymes for the catalytic decomposition of organophosphate nerve agents. A combinatorial library of active site mutants was constructed by randomizing residues His-254, His-257, and Leu-303. The collection of mutant proteins was screened for the ability to hydrolyze a chromogenic analogue of the most toxic stereoisomer of the chemical warfare agent, soman. The mutant H254G/H257W/L303T catalyzed the hydrolysis of the target substrate nearly 3 orders of magnitude faster than the wild-type enzyme. The X-ray crystal structure was solved in the presence and absence of diisopropyl methyl phosphonate. The mutant enzyme was ligated to an additional divalent cation at the active site that was displaced upon the binding of the substrate analogue inhibitor. These studies demonstrate that substantial changes in substrate specificity can be achieved by relatively minor changes to the primary amino acid sequence.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15369336}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15369336 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15369336}}
==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Nerve agent]]
[[Category: Nerve agent]]
[[Category: Tim barrel]]
[[Category: Tim barrel]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:44:22 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:08:54 2008''

Revision as of 23:09, 28 July 2008

Image:1p6c.png

Template:STRUCTURE 1p6c

crystal structure of phosphotriesterase triple mutant H254G/H257W/L303T complexed with diisopropylmethylphosphonate

Template:ABSTRACT PUBMED 15369336

About this Structure

1P6C is a Single protein structure of sequence from Flavobacterium sp.. Full crystallographic information is available from OCA.

Reference

Enhanced degradation of chemical warfare agents through molecular engineering of the phosphotriesterase active site., Hill CM, Li WS, Thoden JB, Holden HM, Raushel FM, J Am Chem Soc. 2003 Jul 30;125(30):8990-1. PMID:15369336

Page seeded by OCA on Tue Jul 29 02:08:54 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1p6c"
Personal tools