2gcc

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[[Image:2gcc.jpg|left|200px]]
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{{STRUCTURE_2gcc| PDB=2gcc | SCENE= }}
{{STRUCTURE_2gcc| PDB=2gcc | SCENE= }}
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'''SOLUTION STRUCTURE OF THE GCC-BOX BINDING DOMAIN, NMR, MINIMIZED MEAN STRUCTURE'''
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===SOLUTION STRUCTURE OF THE GCC-BOX BINDING DOMAIN, NMR, MINIMIZED MEAN STRUCTURE===
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==Overview==
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The 3D solution structure of the GCC-box binding domain of a protein from Arabidopsis thaliana in complex with its target DNA fragment has been determined by heteronuclear multidimensional NMR in combination with simulated annealing and restrained molecular dynamic calculation. The domain consists of a three-stranded anti-parallel beta-sheet and an alpha-helix packed approximately parallel to the beta-sheet. Arginine and tryptophan residues in the beta-sheet are identified to contact eight of the nine consecutive base pairs in the major groove, and at the same time bind to the sugar phosphate backbones. The target DNA bends slightly at the central CG step, thereby allowing the DNA to follow the curvature of the beta-sheet.
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(as it appears on PubMed at http://www.pubmed.gov), where 9736626 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9736626}}
==About this Structure==
==About this Structure==
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2GCC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GCC OCA].
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2GCC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GCC OCA].
==Reference==
==Reference==
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[[Category: Ethlene inducible]]
[[Category: Ethlene inducible]]
[[Category: Transcription factor]]
[[Category: Transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:56:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:30:41 2008''

Revision as of 23:30, 28 July 2008

Image:2gcc.png

Template:STRUCTURE 2gcc

SOLUTION STRUCTURE OF THE GCC-BOX BINDING DOMAIN, NMR, MINIMIZED MEAN STRUCTURE

Template:ABSTRACT PUBMED 9736626

About this Structure

2GCC is a Single protein structure of sequence from Arabidopsis thaliana. Full experimental information is available from OCA.

Reference

A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA., Allen MD, Yamasaki K, Ohme-Takagi M, Tateno M, Suzuki M, EMBO J. 1998 Sep 15;17(18):5484-96. PMID:9736626

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