1z5f

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[[Image:1z5f.gif|left|200px]]
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{{STRUCTURE_1z5f| PDB=1z5f | SCENE= }}
{{STRUCTURE_1z5f| PDB=1z5f | SCENE= }}
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'''Solution Structure of the Cytotoxic RC-RNase 3 with a Pyroglutamate Residue at the N-terminus'''
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===Solution Structure of the Cytotoxic RC-RNase 3 with a Pyroglutamate Residue at the N-terminus===
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==Overview==
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Many proteins and bioactive peptides contain an N-terminal pyroglutamate residue (Pyr1). This residue reduces the susceptibility of the protein to aminopeptidases and often has important functional roles. The antitumor ribonuclease RC-RNase 3 (RNase 3) from oocytes of Rana catesbeiana (bullfrog) is one such protein. We have produced recombinant RNase 3 containing the N-terminal Pyr1 (pRNase 3) and found it to be indistinguishable from the native RNase 3 by mass spectrometry and a variety of other biochemical and immunological criteria. We demonstrated by NMR analysis that the Pyr1 of pRNase 3 forms hydrogen bonds with Lys9 and Ile96 and stabilizes the N-terminal alpha-helix in a rigid conformation. In contrast, the N-terminal alpha-helix becomes flexible and the pKa values of the catalytic residues His10 and His97 altered when Pyr1 formation is blocked by an extra methionine at the N terminus in the recombinant mqRNase 3. Thus, our results provide a mechanistic explanation on the essential role of Pyr1 in maintaining the structural integrity, especially at the N-terminal alpha-helix, and in providing the proper environment for the ionization of His10 and His97 residues for catalysis and cytotoxicity against HeLa cells.
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(as it appears on PubMed at http://www.pubmed.gov), where 16309702 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16309702}}
==About this Structure==
==About this Structure==
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1Z5F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z5F OCA].
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1Z5F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z5F OCA].
==Reference==
==Reference==
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[[Category: Pyroglutamate]]
[[Category: Pyroglutamate]]
[[Category: Ribonuclease]]
[[Category: Ribonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:11:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:11:30 2008''

Revision as of 00:11, 29 July 2008

Image:1z5f.png

Template:STRUCTURE 1z5f

Solution Structure of the Cytotoxic RC-RNase 3 with a Pyroglutamate Residue at the N-terminus

Template:ABSTRACT PUBMED 16309702

About this Structure

1Z5F is a Single protein structure of sequence from Rana catesbeiana. Full experimental information is available from OCA.

Reference

Roles of N-terminal pyroglutamate in maintaining structural integrity and pKa values of catalytic histidine residues in bullfrog ribonuclease 3., Lou YC, Huang YC, Pan YR, Chen C, Liao YD, J Mol Biol. 2006 Jan 20;355(3):409-21. Epub 2005 Nov 10. PMID:16309702

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