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| | {{STRUCTURE_1ulk| PDB=1ulk | SCENE= }} | | {{STRUCTURE_1ulk| PDB=1ulk | SCENE= }} |
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| - | '''Crystal Structure of Pokeweed Lectin-C'''
| + | ===Crystal Structure of Pokeweed Lectin-C=== |
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| - | ==Overview==
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| - | The roots of pokeweed (Phytolacca americana) are known to contain the lectins designated PL-A, PL-B, PL-C, PL-D1, and PL-D2. Of these lectins, the crystal structures of two PLs, the ligand-free PL-C and the complex of PL-D2 with tri-N-acetylchitotriose, have been determined at 1.8A resolution. The polypeptide chains of PL-C and PL-D2 form three and two repetitive chitin-binding domains, respectively. In the crystal structure of the PL-D2 complex, one trisaccharide molecule is shared mainly between two neighboring molecules related to each other by a crystallographic 2(1)-screw axis, and infinite helical chains of complexed molecules are generated by the sharing of ligand molecules. The crystal structure of PL-C reveals that the molecule is a dimer of two identical subunits, whose polypeptide chains are located in a head-to-tail fashion by a molecular 2-fold axis. Three putative carbohydrate-binding sites in each subunit are located in the dimer interface. The dimerization of PL-C is performed through the hydrophobic interactions between the carbohydrate-binding sites of the opposite domains in the dimer, leading to a distinct dimerization mode from that of wheat-germ agglutinin. Three aromatic residues in each carbohydrate-binding site of PL-C are involved in the dimerization. These residues correspond to the residues that interact mainly with the trisaccharide in the PL-D2 complex and appear to mimic the saccharide residues in the complex. Consequently, the present structure of the PL-C dimer has no room for accommodating carbohydrate. The quaternary structure of PL-C formed through these putative carbohydrate-binding residues may lead to the lack of hemagglutinating activity. | + | The line below this paragraph, {{ABSTRACT_PUBMED_14623194}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 14623194 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_14623194}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Lectin]] | | [[Category: Lectin]] |
| | [[Category: Pl-c]] | | [[Category: Pl-c]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:23:36 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:15:00 2008'' |
Revision as of 00:15, 29 July 2008
Template:STRUCTURE 1ulk
Crystal Structure of Pokeweed Lectin-C
Template:ABSTRACT PUBMED 14623194
About this Structure
1ULK is a Single protein structure of sequence from Phytolacca americana. Full crystallographic information is available from OCA.
Reference
Similarity between protein-protein and protein-carbohydrate interactions, revealed by two crystal structures of lectins from the roots of pokeweed., Hayashida M, Fujii T, Hamasu M, Ishiguro M, Hata Y, J Mol Biol. 2003 Nov 28;334(3):551-65. PMID:14623194
Page seeded by OCA on Tue Jul 29 03:15:00 2008
