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2aww

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[[Image:2aww.gif|left|200px]]
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[[Image:2aww.png|left|200px]]
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{{STRUCTURE_2aww| PDB=2aww | SCENE= }}
{{STRUCTURE_2aww| PDB=2aww | SCENE= }}
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'''Synapse associated protein 97 PDZ2 domain variant C378G with C-terminal GluR-A peptide'''
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===Synapse associated protein 97 PDZ2 domain variant C378G with C-terminal GluR-A peptide===
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==Overview==
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Synaptic targeting of GluR-A subunit-containing glutamate receptors involves an interaction with synapse-associated protein 97 (SAP97). The C-terminus of GluR-A, which contains a class I PDZ ligand motif (-x-Ser/Thr-x-phi-COOH where phi is an aliphatic amino acid) associates preferentially with the second PDZ domain of SAP97 (SAP97(PDZ2)). To understand the structural basis of this interaction, we have determined the crystal structures of wild-type and a SAP97(PDZ2) variant in complex with an 18-mer C-terminal peptide (residues 890-907) of GluR-A and of two variant PDZ2 domains in unliganded state at 1.8-2.44 A resolutions. SAP97(PDZ2) folds to a compact globular domain comprising six beta-strands and two alpha-helices, a typical architecture for PDZ domains. In the structure of the peptide complex, only the last four C-terminal residues of the GluR-A are visible, and align as an antiparallel beta-strand in the binding groove of SAP97(PDZ2). The free carboxylate group and the aliphatic side chain of the C-terminal leucine (Leu907), and the hydroxyl group of Thr905 of the GluR-A peptide are engaged in essential class I PDZ interactions. Comparison between the free and complexed structures reveals conformational changes which take place upon peptide binding. The betaAlpha-betaBeta loop moves away from the C-terminal end of alphaB leading to a slight opening of the binding groove, which may better accommodate the peptide ligand. The two conformational states are stabilized by alternative hydrogen bond and coulombic interactions of Lys324 in betaAlpha-betaBeta loop with Asp396 or Thr394 in betaBeta. Results of in vitro binding and immunoprecipitation experiments using a PDZ motif-destroying L907A mutation as well as the insertion of an extra alanine residue between the C-terminal Leu907 and the stop codon are also consistent with a 'classical' type I PDZ interaction between SAP97 and GluR-A C-terminus.
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(as it appears on PubMed at http://www.pubmed.gov), where 17069616 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17069616}}
==About this Structure==
==About this Structure==
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[[Category: Synaptic signaling]]
[[Category: Synaptic signaling]]
[[Category: Trafficking protein]]
[[Category: Trafficking protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:34:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:21:40 2008''

Revision as of 00:21, 29 July 2008

Image:2aww.png

Template:STRUCTURE 2aww

Synapse associated protein 97 PDZ2 domain variant C378G with C-terminal GluR-A peptide

Template:ABSTRACT PUBMED 17069616

About this Structure

2AWW is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the second PDZ domain of SAP97 in complex with a GluR-A C-terminal peptide., von Ossowski I, Oksanen E, von Ossowski L, Cai C, Sundberg M, Goldman A, Keinanen K, FEBS J. 2006 Nov;273(22):5219-29. Epub 2006 Oct 26. PMID:17069616

Page seeded by OCA on Tue Jul 29 03:21:40 2008

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