This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1s4e

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1s4e.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1s4e.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1s4e| PDB=1s4e | SCENE= }}
{{STRUCTURE_1s4e| PDB=1s4e | SCENE= }}
-
'''Pyrococcus furiosus galactokinase in complex with galactose, ADP and magnesium'''
+
===Pyrococcus furiosus galactokinase in complex with galactose, ADP and magnesium===
-
==Overview==
+
<!--
-
Galactokinase (GalK) catalyses the first step of the Leloir pathway of galactose metabolism, the ATP-dependent phosphorylation of galactose to galactose-1-phosphate. In man, defects in galactose metabolism can result in disorders with severe clinical consequences, and deficiencies in galactokinase have been linked with the development of cataracts within the first few months of life. The crystal structure of GalK from Pyrococcus furiosus in complex with MgADP and galactose has been determined to 2.9 A resolution to provide insights into the substrate specificity and catalytic mechanism of the enzyme. The structure consists of two domains with the active site in a cleft at the domain interface. Inspection of the substrate binding pocket identifies the amino acid residues involved in galactose and nucleotide binding and points to both structural and mechanistic similarities with other enzymes of the GHMP kinase superfamily to which GalK belongs. Comparison of the sequence of the Gal3p inducer protein, which is related to GalK and which forms part of the transcriptional activation of the GAL gene cluster in the yeast Saccharomyces cerevisiae, has led to an understanding of the molecular basis of galactose and nucleotide recognition. Finally, the structure has enabled us to further our understanding on the functional consequences of mutations in human GalK which cause galactosemia.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15003454}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15003454 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15003454}}
==About this Structure==
==About this Structure==
Line 36: Line 40:
[[Category: Ghmp kinase superfamily]]
[[Category: Ghmp kinase superfamily]]
[[Category: P-loop]]
[[Category: P-loop]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:16:58 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:22:08 2008''

Revision as of 00:22, 29 July 2008

Image:1s4e.png

Template:STRUCTURE 1s4e

Pyrococcus furiosus galactokinase in complex with galactose, ADP and magnesium

Template:ABSTRACT PUBMED 15003454

About this Structure

1S4E is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.

Reference

Substrate specificity and mechanism from the structure of Pyrococcus furiosus galactokinase., Hartley A, Glynn SE, Barynin V, Baker PJ, Sedelnikova SE, Verhees C, de Geus D, van der Oost J, Timson DJ, Reece RJ, Rice DW, J Mol Biol. 2004 Mar 19;337(2):387-98. PMID:15003454

Page seeded by OCA on Tue Jul 29 03:22:08 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1s4e"
Personal tools