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2d03

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{{STRUCTURE_2d03| PDB=2d03 | SCENE= }}
{{STRUCTURE_2d03| PDB=2d03 | SCENE= }}
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'''Crystal structure of the G91S mutant of the NNA7 Fab'''
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===Crystal structure of the G91S mutant of the NNA7 Fab===
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==Overview==
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The NNA7 Fab antibody fragment recognizes the human N-type blood-group antigen comprised of the N-terminal glycopeptide of glycophorin A (GPA). A mutant form of this Fab fragment, NNA7-G91S, exhibits markedly reduced antigen binding. To provide insight into how these Fab fragments recognize this glycopeptide antigen, the crystal structures of NNA7 and NNA7-G91S were solved and refined to 1.83 and 1.97 A resolution, respectively. Both molecules are composed of the same heavy (H) chain Fd fragment, but each contains a slightly different light (L) chain owing to the G91S substitution. Specifically, the G91S mutation pushes the backbone of the neighboring H chain away from complementarity-determining region 3 (CDR3) of the L-chain variable region, allowing an additional glycerol cryoprotectant molecule to enter the antigen-combining site near the putative location of O-linked glycosylation. Each Fab fragment also possesses a well defined 2-(N-morpholino)ethanesulfonic acid (MES) molecule trapped in its antigen-combining site, as well as a crystallographic symmetry-related molecule comprising an amino-acid sequence that is virtually identical to the N-terminus of GPA. The MES molecule interacts with the H-chain CDR in a manner reminiscent of antibody-carbohydrate complexes. These results suggest a model for recognition of the glycopeptide antigen that accounts for the deleterious effect of the G91S substitution.
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(as it appears on PubMed at http://www.pubmed.gov), where 16204891 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16204891}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystallographic analysis of the NNA7 Fab and proposal for the mode of human blood-group recognition., Xie K, Song SC, Spitalnik SL, Wedekind JE, Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1386-94. Epub 2005, Sep 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16204891 16204891]
Crystallographic analysis of the NNA7 Fab and proposal for the mode of human blood-group recognition., Xie K, Song SC, Spitalnik SL, Wedekind JE, Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1386-94. Epub 2005, Sep 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16204891 16204891]
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Purification, crystallization and X-ray diffraction analysis of a recombinant Fab that recognizes a human blood-group antigen., Song SC, Xie K, Czerwinski M, Spitalnik SL, Wedekind JE, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):788-91. Epub 2004, Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15039587 15039587]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Xie, K.]]
[[Category: Xie, K.]]
[[Category: Antibody]]
[[Category: Antibody]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:27:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:29:50 2008''

Revision as of 00:29, 29 July 2008

Image:2d03.png

Template:STRUCTURE 2d03

Crystal structure of the G91S mutant of the NNA7 Fab

Template:ABSTRACT PUBMED 16204891

About this Structure

2D03 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystallographic analysis of the NNA7 Fab and proposal for the mode of human blood-group recognition., Xie K, Song SC, Spitalnik SL, Wedekind JE, Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1386-94. Epub 2005, Sep 28. PMID:16204891

Purification, crystallization and X-ray diffraction analysis of a recombinant Fab that recognizes a human blood-group antigen., Song SC, Xie K, Czerwinski M, Spitalnik SL, Wedekind JE, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):788-91. Epub 2004, Mar 23. PMID:15039587

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