This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1n7f

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1n7f.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1n7f.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1n7f| PDB=1n7f | SCENE= }}
{{STRUCTURE_1n7f| PDB=1n7f | SCENE= }}
-
'''Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide'''
+
===Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide===
-
==Overview==
+
<!--
-
PDZ domains bind to short segments within target proteins in a sequence-specific fashion. Glutamate receptor-interacting protein (GRIP)/ABP family proteins contain six to seven PDZ domains and interact via the sixth PDZ domain (class II) with the C termini of various proteins including liprin-alpha. In addition the PDZ456 domain mediates the formation of homo- and heteromultimers of GRIP proteins. To better understand the structural basis of peptide recognition by a class II PDZ domain and PDZ-mediated multimerization, we determined the crystal structures of the GRIP1 PDZ6 domain alone and in complex with a synthetic C-terminal octapeptide of human liprin-alpha at resolutions of 1.5 and 1.8 A, respectively. Remarkably, unlike other class II PDZ domains, Ile-736 at alphaB5 rather than conserved Leu-732 at alphaB1 makes a direct hydrophobic contact with the side chain of the Tyr at the -2 position of the ligand. Moreover, the peptide-bound structure of PDZ6 shows a slight reorientation of helix alphaB, indicating that the second hydrophobic pocket undergoes a conformational adaptation to accommodate the bulkiness of the Tyr side chain, and forms an antiparallel dimer through an interface located at a site distal to the peptide-binding groove. This configuration may enable formation of GRIP multimers and efficient clustering of GRIP-binding proteins.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12493751}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12493751 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12493751}}
==About this Structure==
==About this Structure==
Line 33: Line 37:
[[Category: Liprin]]
[[Category: Liprin]]
[[Category: Pdz]]
[[Category: Pdz]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:11:14 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:39:06 2008''

Revision as of 00:39, 29 July 2008

Image:1n7f.png

Template:STRUCTURE 1n7f

Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide

Template:ABSTRACT PUBMED 12493751

About this Structure

1N7F is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Crystal structure of GRIP1 PDZ6-peptide complex reveals the structural basis for class II PDZ target recognition and PDZ domain-mediated multimerization., Im YJ, Park SH, Rho SH, Lee JH, Kang GB, Sheng M, Kim E, Eom SH, J Biol Chem. 2003 Mar 7;278(10):8501-7. Epub 2002 Dec 18. PMID:12493751

Page seeded by OCA on Tue Jul 29 03:39:06 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n7f"
Personal tools