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1yl4

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{{STRUCTURE_1yl4| PDB=1yl4 | SCENE= }}
{{STRUCTURE_1yl4| PDB=1yl4 | SCENE= }}
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'''Crystal structure of 70S ribosome with thrS operator and tRNAs. 30S subunit. The coordinates for the 50S subunit are in the pdb entry 1YL3'''
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===Crystal structure of 70S ribosome with thrS operator and tRNAs. 30S subunit. The coordinates for the 50S subunit are in the pdb entry 1YL3===
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==Overview==
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The ribosome of Thermus thermophilus was cocrystallized with initiator transfer RNA (tRNA) and a structured messenger RNA (mRNA) carrying a translational operator. The path of the mRNA was defined at 5.5 angstroms resolution by comparing it with either the crystal structure of the same ribosomal complex lacking mRNA or with an unstructured mRNA. A precise ribosomal environment positions the operator stem-loop structure perpendicular to the surface of the ribosome on the platform of the 30S subunit. The binding of the operator and of the initiator tRNA occurs on the ribosome with an unoccupied tRNA exit site, which is expected for an initiation complex. The positioning of the regulatory domain of the operator relative to the ribosome elucidates the molecular mechanism by which the bound repressor switches off translation. Our data suggest a general way in which mRNA control elements must be placed on the ribosome to perform their regulatory task.
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(as it appears on PubMed at http://www.pubmed.gov), where 15802605 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15802605}}
==About this Structure==
==About this Structure==
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[[Category: Ribosome]]
[[Category: Ribosome]]
[[Category: Translational operator]]
[[Category: Translational operator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:27:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:40:22 2008''

Revision as of 00:40, 29 July 2008

Image:1yl4.png

Template:STRUCTURE 1yl4

Crystal structure of 70S ribosome with thrS operator and tRNAs. 30S subunit. The coordinates for the 50S subunit are in the pdb entry 1YL3

Template:ABSTRACT PUBMED 15802605

About this Structure

1YL4 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Translational operator of mRNA on the ribosome: how repressor proteins exclude ribosome binding., Jenner L, Romby P, Rees B, Schulze-Briese C, Springer M, Ehresmann C, Ehresmann B, Moras D, Yusupova G, Yusupov M, Science. 2005 Apr 1;308(5718):120-3. PMID:15802605

Page seeded by OCA on Tue Jul 29 03:40:22 2008

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