2djm

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[[Image:2djm.gif|left|200px]]
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{{STRUCTURE_2djm| PDB=2djm | SCENE= }}
{{STRUCTURE_2djm| PDB=2djm | SCENE= }}
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'''Solution structure of N-terminal starch-binding domain of glucoamylase from Rhizopus oryzae'''
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===Solution structure of N-terminal starch-binding domain of glucoamylase from Rhizopus oryzae===
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==Overview==
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CBMs (carbohydrate-binding modules) function independently to assist carbohydrate-active enzymes. Family 21 CBMs contain approx. 100 amino acid residues, and some members have starchbinding functions or glycogen-binding activities. We report here the first structure of a family 21 CBM from the SBD (starch-binding domain) of Rhizopus oryzae glucoamylase (RoCBM21) determined by NMR spectroscopy. This CBM has a beta-sandwich fold with an immunoglobulin-like structure. Ligand-binding properties of RoCBM21 were analysed by chemical-shift perturbations and automated docking. Structural comparisons with previously reported SBDs revealed two types of topologies, namely type I and type II, with CBM20, CBM25, CBM26 and CBM41 showing type I topology, with CBM21 and CBM34 showing type II topology. According to the chemical-shift perturbations, RoCBM21 contains two ligand-binding sites. Residues in site II are similar to those found in the family 20 CBM from Aspergillus niger glucoamylase (AnCBM20). Site I, however, is embedded in a region with unique sequence motifs only found in some members of CBM21s. Additionally, docking of beta-cyclodextrin and malto-oligosaccharides highlights that side chains of Y83 and W47 (one-letter amino acid code) form the central part of the conserved binding platform in the SBD. The structure of RoCBM21 provides the first direct evidence of the structural features and the basis for protein-carbohydrate recognition from an SBD of CBM21.
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{{ABSTRACT_PUBMED_17117925}}
==About this Structure==
==About this Structure==
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2DJM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhizopus_oryzae Rhizopus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DJM OCA].
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2DJM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhizopus_oryzae Rhizopus oryzae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DJM OCA].
==Reference==
==Reference==
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[[Category: Carbohydrate binding]]
[[Category: Carbohydrate binding]]
[[Category: Strach binding]]
[[Category: Strach binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:33:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:45:17 2008''

Revision as of 00:45, 29 July 2008

Image:2djm.png

Template:STRUCTURE 2djm

Solution structure of N-terminal starch-binding domain of glucoamylase from Rhizopus oryzae

Template:ABSTRACT PUBMED 17117925

About this Structure

2DJM is a Single protein structure of sequence from Rhizopus oryzae. Full experimental information is available from OCA.

Reference

Solution structure of family 21 carbohydrate-binding module from Rhizopus oryzae glucoamylase., Liu YN, Lai YT, Chou WI, Chang MD, Lyu PC, Biochem J. 2007 Apr 1;403(1):21-30. PMID:17117925

Page seeded by OCA on Tue Jul 29 03:45:17 2008

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