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1w3p

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{{STRUCTURE_1w3p| PDB=1w3p | SCENE= }}
{{STRUCTURE_1w3p| PDB=1w3p | SCENE= }}
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'''NIMA FROM D. RADIODURANS WITH A HIS71-PYRUVATE RESIDUE'''
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===NIMA FROM D. RADIODURANS WITH A HIS71-PYRUVATE RESIDUE===
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==Overview==
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5-Nitroimidazole-based antibiotics are compounds extensively used for treating infections in humans and animals caused by several important pathogens. They are administered as prodrugs, and their activation depends upon an anaerobic 1-electron reduction of the nitro group by a reduction pathway in the cells. Bacterial resistance toward these drugs is thought to be caused by decreased drug uptake and/or an altered reduction efficiency. One class of resistant strains, identified in Bacteroides, has been shown to carry Nim genes (NimA, -B, -C, -D, and -E), which encode for reductases that convert the nitro group on the antibiotic into a non-bactericidal amine. In this paper, we have described the crystal structure of NimA from Deinococcus radiodurans (drNimA) at 1.6 A resolution. We have shown that drNimA is a homodimer in which each monomer adopts a beta-barrel fold. We have identified the catalytically important His-71 along with the cofactor pyruvate and antibiotic binding sites, all of which are found at the monomer-monomer interface. We have reported three additional crystal structures of drNimA, one in which the antibiotic metronidazole is bound to the protein, one with pyruvate covalently bound to His-71, and one with lactate covalently bound to His-71. Based on these structures, a reaction mechanism has been proposed in which the 2-electron reduction of the antibiotic prevents accumulation of the toxic nitro radical. This mechanism suggests that Nim proteins form a new class of reductases, conferring resistance against 5-nitroimidazole-based antibiotics.
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{{ABSTRACT_PUBMED_15492014}}
==About this Structure==
==About this Structure==
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[[Category: Deinococcus radioduran]]
[[Category: Deinococcus radioduran]]
[[Category: Nim gene]]
[[Category: Nim gene]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:01:05 2008''

Revision as of 02:01, 29 July 2008

Image:1w3p.png

Template:STRUCTURE 1w3p

NIMA FROM D. RADIODURANS WITH A HIS71-PYRUVATE RESIDUE

Template:ABSTRACT PUBMED 15492014

About this Structure

1W3P is a Single protein structure of sequence from Deinococcus radiodurans. Full crystallographic information is available from OCA.

Reference

Structural basis of 5-nitroimidazole antibiotic resistance: the crystal structure of NimA from Deinococcus radiodurans., Leiros HK, Kozielski-Stuhrmann S, Kapp U, Terradot L, Leonard GA, McSweeney SM, J Biol Chem. 2004 Dec 31;279(53):55840-9. Epub 2004 Oct 18. PMID:15492014

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