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1sql

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{{STRUCTURE_1sql| PDB=1sql | SCENE= }}
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'''Crystal structure of 7,8-dihydroneopterin aldolase in complex with guanine'''
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===Crystal structure of 7,8-dihydroneopterin aldolase in complex with guanine===
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==Overview==
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Dihydroneopterin aldolase (DHNA) catalyses a retroaldol reaction yielding 6-hydroxymethyl-7,8-dihydropterin, a biosynthetic precursor of the vitamin, tetrahydrofolate. The enzyme is a potential target for antimicrobial and anti-parasite chemotherapy. A gene specifying a dihydroneopterin aldolase from Arabidopsis thaliana was expressed in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity and crystallised using polyethylenglycol as the precipitating agent. The crystal structure was solved by X-ray diffraction analysis at 2.2A resolution. The enzyme forms a D(4)-symmetric homooctamer. Each polypeptide chain is folded into a single domain comprising an antiparallel four-stranded beta-sheet and two long alpha-helices. Four monomers are arranged in a tetrameric ring, and two of these rings form a hollow cylinder. Well defined purine derivatives are found at all eight topologically equivalent active sites. The subunit fold of the enzyme is related to substructures of dihydroneopterin triphosphate epimerase, GTP cyclohydrolase I, and pyruvoyltetrahydropterin synthase, which are all involved in the biosynthesis of pteridine type cofactors, and to urate oxidase, although some members of that superfamily have no detectable sequence similarity. Due to structural and mechanistical differences of DHNA in comparison with class I and class II aldolases, a new aldolase class is proposed.
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{{ABSTRACT_PUBMED_15165863}}
==About this Structure==
==About this Structure==
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[[Category: Schiff base]]
[[Category: Schiff base]]
[[Category: Tetrahydrofolate biosynthesis]]
[[Category: Tetrahydrofolate biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:01:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:36:11 2008''

Revision as of 02:36, 29 July 2008

Image:1sql.png

Template:STRUCTURE 1sql

Crystal structure of 7,8-dihydroneopterin aldolase in complex with guanine

Template:ABSTRACT PUBMED 15165863

About this Structure

1SQL is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

Reference

Biosynthesis of tetrahydrofolate in plants: crystal structure of 7,8-dihydroneopterin aldolase from Arabidopsis thaliana reveals a novel adolase class., Bauer S, Schott AK, Illarionova V, Bacher A, Huber R, Fischer M, J Mol Biol. 2004 Jun 11;339(4):967-79. PMID:15165863

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