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1sub

From Proteopedia

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[[Image:1sub.jpg|left|200px]]
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{{STRUCTURE_1sub| PDB=1sub | SCENE= }}
{{STRUCTURE_1sub| PDB=1sub | SCENE= }}
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'''CALCIUM-INDEPENDENT SUBTILISIN BY DESIGN'''
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===CALCIUM-INDEPENDENT SUBTILISIN BY DESIGN===
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==Overview==
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A version of subtilisin BPN' lacking the high affinity calcium site (site A) has been produced through genetic engineering methods, and its crystal structure refined at 1.8 A resolution. This protein and the corresponding version containing the calcium A site are described and compared. The deletion of residues 75-83 was made in the context of four site-specific replacements previously shown to stabilize subtilisin. The helix that in wild type is interrupted by the calcium binding loop, is continuous in the deletion mutant, with normal geometry. A few residues adjacent to the loop, principally those that were involved in calcium coordination, are repositioned and/or destabilized by the deletion. Because refolding is greatly facilitated by the absence of the Ca-loop, this protein offers a new vehicle for analysis and dissection of the folding reaction. This is among the largest internal changes to a protein to be described at atomic resolution.
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The line below this paragraph, {{ABSTRACT_PUBMED_8332608}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 8332608 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8332608}}
==About this Structure==
==About this Structure==
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[[Category: Gallagher, T.]]
[[Category: Gallagher, T.]]
[[Category: Gilliland, G L.]]
[[Category: Gilliland, G L.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:08:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:38:17 2008''

Revision as of 03:38, 29 July 2008

Image:1sub.png

Template:STRUCTURE 1sub

CALCIUM-INDEPENDENT SUBTILISIN BY DESIGN

Template:ABSTRACT PUBMED 8332608

About this Structure

1SUB is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.

Reference

Calcium-independent subtilisin by design., Gallagher T, Bryan P, Gilliland GL, Proteins. 1993 Jun;16(2):205-13. PMID:8332608

Page seeded by OCA on Tue Jul 29 06:38:17 2008

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