2i7t

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{{STRUCTURE_2i7t| PDB=2i7t | SCENE= }}
{{STRUCTURE_2i7t| PDB=2i7t | SCENE= }}
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'''Structure of human CPSF-73'''
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===Structure of human CPSF-73===
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==Overview==
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Most eukaryotic messenger RNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3'-end. Despite the characterization of many proteins that are required for the cleavage reaction, the identity of the endonuclease is not known. Recent analyses indicated that the 73-kDa subunit of cleavage and polyadenylation specificity factor (CPSF-73) might be the endonuclease for this and related reactions, although no direct data confirmed this. Here we report the crystal structures of human CPSF-73 at 2.1 A resolution, complexed with zinc ions and a sulphate that might mimic the phosphate group of the substrate, and the related yeast protein CPSF-100 (Ydh1) at 2.5 A resolution. Both CPSF-73 and CPSF-100 contain two domains, a metallo-beta-lactamase domain and a novel beta-CASP (named for metallo-beta-lactamase, CPSF, Artemis, Snm1, Pso2) domain. The active site of CPSF-73, with two zinc ions, is located at the interface of the two domains. Purified recombinant CPSF-73 possesses RNA endonuclease activity, and mutations that disrupt zinc binding in the active site abolish this activity. Our studies provide the first direct experimental evidence that CPSF-73 is the pre-mRNA 3'-end-processing endonuclease.
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{{ABSTRACT_PUBMED_17128255}}
==About this Structure==
==About this Structure==
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[[Category: Polyadenylation]]
[[Category: Polyadenylation]]
[[Category: Pre-mrna processing]]
[[Category: Pre-mrna processing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:11:17 2008''

Revision as of 04:11, 29 July 2008

Image:2i7t.png

Template:STRUCTURE 2i7t

Structure of human CPSF-73

Template:ABSTRACT PUBMED 17128255

About this Structure

2I7T is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease., Mandel CR, Kaneko S, Zhang H, Gebauer D, Vethantham V, Manley JL, Tong L, Nature. 2006 Dec 14;444(7121):953-6. Epub 2006 Nov 26. PMID:17128255

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