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2nq9

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[[Image:2nq9.gif|left|200px]]
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{{STRUCTURE_2nq9| PDB=2nq9 | SCENE= }}
{{STRUCTURE_2nq9| PDB=2nq9 | SCENE= }}
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'''High resolution crystal structure of Escherichia coli endonuclease IV (Endo IV) Y72A mutant bound to damaged DNA'''
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===High resolution crystal structure of Escherichia coli endonuclease IV (Endo IV) Y72A mutant bound to damaged DNA===
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==Overview==
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Escherichia coli endonuclease IV is an archetype for an abasic or apurinic-apyrimidinic endonuclease superfamily crucial for DNA base excision repair. Here biochemical, mutational and crystallographic characterizations reveal a three-metal ion mechanism for damage binding and incision. The 1.10-A resolution DNA-free and the 2.45-A resolution DNA-substrate complex structures capture substrate stabilization by Arg37 and reveal a distorted Zn(3)-ligand arrangement that reverts, after catalysis, to an ideal geometry suitable to hold rather than release cleaved DNA product. The 1.45-A resolution DNA-product complex structure shows how Tyr72 caps the active site, tunes its dielectric environment and promotes catalysis by Glu261-activated hydroxide, bound to two Zn(2+) ions throughout catalysis. These structural, mutagenesis and biochemical results suggest general requirements for abasic site removal in contrast to features specific to the distinct endonuclease IV alpha-beta triose phosphate isomerase (TIM) barrel and APE1 four-layer alpha-beta folds of the apurinic-apyrimidinic endonuclease families.
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The line below this paragraph, {{ABSTRACT_PUBMED_18408731}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 18408731 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18408731}}
==About this Structure==
==About this Structure==
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[[Category: Tim-barrel]]
[[Category: Tim-barrel]]
[[Category: Trinuclear zn active site]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:25:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:26:30 2008''

Revision as of 04:26, 29 July 2008

Image:2nq9.png

Template:STRUCTURE 2nq9

High resolution crystal structure of Escherichia coli endonuclease IV (Endo IV) Y72A mutant bound to damaged DNA

Template:ABSTRACT PUBMED 18408731

About this Structure

2NQ9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

DNA apurinic-apyrimidinic site binding and excision by endonuclease IV., Garcin ED, Hosfield DJ, Desai SA, Haas BJ, Bjoras M, Cunningham RP, Tainer JA, Nat Struct Mol Biol. 2008 Apr 13;. PMID:18408731

Page seeded by OCA on Tue Jul 29 07:26:30 2008

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