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2glg

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[[Image:2glg.gif|left|200px]]
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[[Image:2glg.png|left|200px]]
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{{STRUCTURE_2glg| PDB=2glg | SCENE= }}
{{STRUCTURE_2glg| PDB=2glg | SCENE= }}
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'''NMR structure of the [L23,A24]-sCT mutant'''
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===NMR structure of the [L23,A24]-sCT mutant===
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==Overview==
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Salmon calcitonin (sCT) forms an amphipathic helix in the region 9-19, with the C-terminal decapeptide interacting with the helix (Amodeo, P., Motta, A., Strazzullo, G., Castiglione Morelli, M. A. (1999) J. Biomol. NMR 13, 161-174). To uncover the structural requirements for the hormone bioactivity, we investigated several sCT analogs. They were designed so as to alter the length of the central helix by removal and/or replacement of flanking residues and by selectively mutating or deleting residues inside the helix. The helix content was assessed by circular dichroism and NMR spectroscopies; the receptor binding affinity in human breast cancer cell line T 47D and the in vivo hypocalcemic activity were also evaluated. In particular, by NMR spectroscopy and molecular dynamics calculations we studied Leu(23),Ala(24)-sCT in which Pro(23) and Arg(24) were replaced by helix inducing residues. Compared with sCT, it assumes a longer amphipathic alpha-helix, with decreased binding affinity and one-fifth of the hypocalcemic activity, therefore supporting the idea of a relationship between a definite helix length and bioactivity. From the analysis of other sCT mutants, we inferred that the correct helix length is located in the 9-19 region and requires long range interactions and the presence of specific regions of residues within the sequence for high binding affinity and hypocalcemic activity. Taken together, the structural and biological data identify well defined structural parameters of the helix for sCT bioactivity.
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(as it appears on PubMed at http://www.pubmed.gov), where 16766525 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16766525}}
==About this Structure==
==About this Structure==
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2GLG is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GLG OCA].
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2GLG is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GLG OCA].
==Reference==
==Reference==
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[[Category: Nakamuta, H.]]
[[Category: Nakamuta, H.]]
[[Category: A-helix]]
[[Category: A-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:14:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:32:28 2008''

Revision as of 04:32, 29 July 2008

Image:2glg.png

Template:STRUCTURE 2glg

NMR structure of the [L23,A24]-sCT mutant

Template:ABSTRACT PUBMED 16766525

About this Structure

2GLG is a Single protein structure. Full experimental information is available from OCA.

Reference

Structural determinants of salmon calcitonin bioactivity: the role of the Leu-based amphipathic alpha-helix., Andreotti G, Mendez BL, Amodeo P, Morelli MA, Nakamuta H, Motta A, J Biol Chem. 2006 Aug 25;281(34):24193-203. Epub 2006 Jun 9. PMID:16766525

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