This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2boi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2boi.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2boi.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2boi| PDB=2boi | SCENE= }}
{{STRUCTURE_2boi| PDB=2boi | SCENE= }}
-
'''1.1A STRUCTURE OF CHROMOBACTERIUM VIOLACEUM LECTIN CV2L IN COMPLEX WITH ALPHA-METHYL-FUCOSIDE'''
+
===1.1A STRUCTURE OF CHROMOBACTERIUM VIOLACEUM LECTIN CV2L IN COMPLEX WITH ALPHA-METHYL-FUCOSIDE===
-
==Overview==
+
<!--
-
The purple pigmented bacterium Chromobacterium violaceum is a dominant component of tropical soil microbiota that can cause rare but fatal septicaemia in humans. Its sequenced genome provides insight into the abundant potential of this organism for biotechnological and pharmaceutical applications and allowed an ORF encoding a protein that is 60% identical to the fucose binding lectin (PA-IIL) from Pseudomonas aeruginosa and the mannose binding lectin (RS-IIL) from Ralstonia solanacearum to be identified. The lectin, CV-IIL, has recently been purified from C. violaceum [Zinger-Yosovich, K., Sudakevitz, D., Imberty, A., Garber, N. C., and Gilboa-Garber, N. (2006) Microbiology 152, 457-463] and has been confirmed to be a tetramer with subunit size of 11.86 kDa and a binding preference for fucose. We describe here the cloning of CV-IIL and its expression as a recombinant protein. A complete structure-function characterization has been made in an effort to analyze the specificity and affinity of CV-IIL for fucose and mannose. Crystal structures of CV-IIL complexes with monosaccharides have yielded the molecular basis of the specificity. Each monomer contains two close calcium cations that mediate the binding of the monosaccharides, which occurs in different orientations for fucose and mannose. The thermodynamics of binding has been analyzed by titration microcalorimetry, giving dissociation constants of 1.7 and 19 microM for alpha-methyl fucoside and alpha-methyl mannoside, respectively. Further analysis demonstrated a strongly favorable entropy term that is unusual in carbohydrate binding. A comparison with both PA-IIL and RS-IIL, which have binding preferences for fucose and mannose, respectively, yielded insights into the monosaccharide specificity of this important class of soluble bacterial lectins.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16768446}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16768446 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16768446}}
==About this Structure==
==About this Structure==
Line 35: Line 39:
[[Category: Lectin]]
[[Category: Lectin]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:34:41 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:45:22 2008''

Revision as of 04:45, 29 July 2008

Image:2boi.png

Template:STRUCTURE 2boi

1.1A STRUCTURE OF CHROMOBACTERIUM VIOLACEUM LECTIN CV2L IN COMPLEX WITH ALPHA-METHYL-FUCOSIDE

Template:ABSTRACT PUBMED 16768446

About this Structure

2BOI is a Single protein structure of sequence from Chromobacterium violaceum. Full crystallographic information is available from OCA.

Reference

Unusual entropy-driven affinity of Chromobacterium violaceum lectin CV-IIL toward fucose and mannose., Pokorna M, Cioci G, Perret S, Rebuffet E, Kostlanova N, Adam J, Gilboa-Garber N, Mitchell EP, Imberty A, Wimmerova M, Biochemistry. 2006 Jun 20;45(24):7501-10. PMID:16768446

Page seeded by OCA on Tue Jul 29 07:45:22 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2boi"
Personal tools