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1qyp

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{{STRUCTURE_1qyp| PDB=1qyp | SCENE= }}
{{STRUCTURE_1qyp| PDB=1qyp | SCENE= }}
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'''THERMOCOCCUS CELER RPB9, NMR, 25 STRUCTURES'''
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===THERMOCOCCUS CELER RPB9, NMR, 25 STRUCTURES===
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==Overview==
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BACKGROUND: Transcriptional initiation and elongation provide control points in gene expression. Eukaryotic RNA polymerase II subunit 9 (RPB9) regulates start-site selection and elongational arrest. RPB9 contains Cys4 Zn(2+)-binding motifs which are conserved in archaea and homologous to those of the general transcription factors TFIIB and TFIIS. RESULTS: The structure of an RPB9 domain from the hyperthermophilic archaeon Thermococcus celer was determined at high resolution by NMR spectroscopy. The structure consists of an apical tetrahedral Zn(2+)-binding site, central beta sheet and disordered loop. Although the structure lacks a globular hydrophobic core, the two surfaces of the beta sheet each contain well ordered aromatic rings engaged in serial edge-to-face interactions. Basic sidechains are clustered near the Zn(2+)-binding site. The disordered loop contains sidechains conserved in TFIIS, including acidic residues essential for the stimulation of transcriptional elongation. CONCLUSIONS: The planar architecture of the RPB9 zinc ribbon-distinct from that of a conventional globular domain-can accommodate significant differences in the alignment of polar, non-polar and charged sidechains. Such divergence is associated with local and non-local changes in structure. The RPB9 structure is distinguished by a fourth beta strand (extending the central beta sheet) in a well ordered N-terminal segment and also differs from TFIIS (but not TFIIB) in the orientation of its apical Zn(2+)-binding site. Cys4 Zn(2+)-binding sites with distinct patterns of polar, non-polar and charged residues are conserved among unrelated RNAP subunits and predicted to form variant zinc ribbons.
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{{ABSTRACT_PUBMED_9634694}}
==About this Structure==
==About this Structure==
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1QYP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermococcus_celer Thermococcus celer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYP OCA].
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1QYP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermococcus_celer Thermococcus celer]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYP OCA].
==Reference==
==Reference==
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[[Category: Transcription]]
[[Category: Transcription]]
[[Category: Zn ribbon]]
[[Category: Zn ribbon]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:51:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:00:13 2008''

Revision as of 05:00, 29 July 2008

Image:1qyp.png

Template:STRUCTURE 1qyp

THERMOCOCCUS CELER RPB9, NMR, 25 STRUCTURES

Template:ABSTRACT PUBMED 9634694

About this Structure

1QYP is a Single protein structure of sequence from Thermococcus celer. Full experimental information is available from OCA.

Reference

High-resolution structure of an archaeal zinc ribbon defines a general architectural motif in eukaryotic RNA polymerases., Wang B, Jones DN, Kaine BP, Weiss MA, Structure. 1998 May 15;6(5):555-69. PMID:9634694

Page seeded by OCA on Tue Jul 29 08:00:13 2008

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